We narrowed to 14,728 results for: EGF

1. EK0500 SFFV-mCherry-SGc(RPLP1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191184

   Purpose

   Expression of RPLP1 sensor in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:RPLP1.s1:EGFP-3xMS2
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)

   Plasmid

   #191177

   Purpose

   Mammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:GAPDH.s1:EGFP (frameshifted)
   

   Expression

   Mammalian

   Mutation

   EGFP is frameshifted

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191183

   Purpose

   Expression of PPIB sensor in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:PPIB.s1:EGFP-3xMS2
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

   Plasmid

   #185490

   Purpose

   CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

   Depositor

   Benjamin Kleinstiver, Raul Perez-Jimenez

   Article

   Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

   Insert

   human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-eGFP

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV and T7

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFJ2306 - [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

   Plasmid

   #159848

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p)

   Plasmid

   #70194

   Purpose

   Derived from 21 with silent mutations to make BsaI, BbsI compatible

   Depositor

   Richard Murray

   Article

   Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

   Insert

   deGFP
   

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP

   Plasmid

   #122956

   Purpose

   Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expression

   Depositor

   Pierre Vanderhaeghen

   Article

   Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

   Insert

   NOTCH2NL-delta C terminus (NOTCH2NLB Human)
   

   Use

   Lentiviral

   Tags

   6xhis tag

   Mutation

   deletion of C terminus from full length NOTCH2NLB

   Promoter

   CAG promoter

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. EK0656 SFFV-mCherry-SGc(70587.os4)cSG-EGFP-(3xMS2)

   Plasmid

   #247269

   Purpose

   modulADAR sensor against sythetic target transcript (syn GluR-B stemloop)

   Depositor

   Xiaojing Gao

   Article

   Kolber et al bioRxiv 2025.09.18.677213

   Insert

   mCherry-s70587.os4-EGFP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. L4791 VEGFR2 NTEVp chain (75S NTEVp mutant) in PolyTX-mNeonGreen

   Plasmid

   #244172

   Purpose

   Constitutive expression of a MESA NTEVp chain encoding (N to C): VEGFR2SS-3xFLAG-VEGFR2ECD-VEGFR2TMD-NTEVp(75S)

   Depositor

   Joshua Leonard

   Article

   Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

   Insert

   MESA NTEVp chain with human VEGFR2 SS, ECD and TMD, and NTEVp (75S) (KDR Human, Synthetic)
   

   Use

   Synthetic Biology

   Tags

   VEGFR2 signal peptide - 3xFLAG

   Expression

   Mammalian

   Mutation

   NTEVp_75S

   Promoter

   hEF1a

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-eCas9-T2A-EGFP-U6-gRNA targeting Nrl-no ITR and f1 ori

    Plasmid

    #234737

    Purpose

    All-in-one CRISPR/Cas9 vector encodes high-fidelity eSpCas9 and a gRNA targeting Nrl, efficiently reprogramming rod precursors into cone-like cells in the mouse retina.

    Depositor

    Kirill Martemyanov

    Article

    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

    Insert

    Nrl (Nrl Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV hU6-VEGFR2#3 sgRNA Nestin-dCas9-KRAB-T2a-GFP

    Plasmid

    #196990

    Purpose

    Derived from pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP with KDR sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

    Insert

    VEGFR2
    

    Use

    CRISPR and Lentiviral

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. LSM075 SFFV-mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP (FLP-IN)

    Plasmid

    #233439

    Purpose

    SFFV driven LIDAR reporter with 2 tandem substrate stem loops

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    mCherry-SGc(stem-1(3xMS2)x2)cSG-EGFP
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST (W18A/L21A; deltaLIR)

    Plasmid

    #223766

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    BNIP3 soluble part (BNIP3 Human)
    

    Tags

    EGFP-TEV-GST

    Expression

    Insect

    Mutation

    W18A/L21A; deletion of LIR

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9

    Plasmid

    #231910

    Purpose

    For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcBac3-OMeYRS-8xYtR-EGFP-3TAA-40TGA-152TAG-TEV-3xUAA

    Plasmid

    #228787

    Purpose

    EcTyr aaRS/tRNA system for TAG suppression with EGFP containing three stop codons for triple suppression

    Depositor

    Abhishek Chatterjee

    Article

    Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

    Inserts

    EcTyrRS
    EcYtR
    EGFP***
    TEV protease
    

    Use

    Baculoviral

    Expression

    Mammalian

    Mutation

    TAG suppressor

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

    Plasmid

    #216871

    Purpose

    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

    Depositor

    Ori Bar-Nur

    Article

    Lenardic et al J Clin Invest. 2024 May 7:e166998. doi:

    Insert

    Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)
    

    Use

    CRISPR

    Mutation

    mdx mutation in mouse dystrophin

    Available Since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A)

    Plasmid

    #218636

    Purpose

    Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    H781E, Q782R, F785A

    Promoter

    UBC

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

    Plasmid

    #218635

    Purpose

    Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Lentiviral

    Tags

    APEX2-3xFLAG

    Expression

    Mammalian

    Mutation

    N1008R, R1009E, E1010R, R1013E

    Promoter

    UBC

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A_S

    Plasmid

    #147515

    Purpose

    Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A (gw Human, Nematode)
    

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A_R

    Plasmid

    #147389

    Purpose

    Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    CeAIN2_254-400-DmGW182_635-1384-M2WA-CIM1mut-F961A (gw Nematode, Fly)
    

    Expression

    Insect

    Mutation

    Deletion of 2 AA (SD) at position 378-379 compare…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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