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tdEos-Farnesyl-5

Plasmid

#57613

Purpose

Localization: Plasma Membrane, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Farnesyl (HRAS Human)

Tags

tdEos

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pEMS2177

Plasmid

#111892

Purpose

High copy number plasmid containing Ple344 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple344 (TUBB3 Human)

Use

Unspecified

Promoter

Ple344 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2175

Plasmid

#111890

Purpose

High copy number plasmid containing Ple342 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple342 (TUBB3 Human)

Use

Unspecified

Promoter

Ple342 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2176

Plasmid

#111891

Purpose

High copy number plasmid containing Ple343 (TUBB3 MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple343 (TUBB3 Human)

Use

Unspecified

Promoter

Ple343 (TUBB3 MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pSBbi-Pur-mCerulean3-spacer-cpVenus[E172]

Plasmid

#125204

Purpose

SB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET low control

Depositor

Eric Davis

Article

Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

Insert

mCerulean3-spacer-cpVenus[E172]

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1984

Plasmid

#49115

Purpose

AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple253-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

PITX3

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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miniSOG-Vimentin-7

Plasmid

#57779

Purpose

Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Vimentin (VIM Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAC4 Grb14-PIRSH2

Plasmid

#46438

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb14 (GRB14 Human)

Tags

AviTag, GST, and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 341-540

Promoter

Trc

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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Major Sat-BUB1

Plasmid

#133350

Purpose

Express a TALE construct that recognizes major satellite repeats fused to mClover and the kinase domain of mouse BUB1 (aa 672-1058) at the C terminus

Depositor

Michael Lampson

Article

Akera et al Cell. 2019 Aug 22;178(5):1132-1144.e10.

Insert

BUB1 (Bub1 Mouse)

Tags

mClover

Expression

Mammalian

Mutation

deleted amino acids 1-671

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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mPapaya1-Actin-C-18

Plasmid

#56400

Purpose

Localization: Actin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Actin (ACTB Human)

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pEMS2276

Plasmid

#111894

Purpose

High Copy number plasmid containing Ple346 (NEFM MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple346 (NEFM Human)

Use

Unspecified

Promoter

Ple346 (NEFM MiniPromoter)

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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SL534

Plasmid

#49940

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD RH-1 (RHOXF2 Human)

Use

TALEN

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only
pGEX Grb7-PIR-SH2

Plasmid

#46443

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Grb7 (GRB7 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 334-532

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX HSH2-SH2

Plasmid

#46446

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

HSH2 (HSH2D Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-134

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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mRuby2-Alpha-Actinin-19

Plasmid

#55890

Purpose

Localization: Alpha Actinin, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Alpha-Actinin (ACTN1 Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2058

Plasmid

#49136

Purpose

AAV plasmid with CAGGS promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-emGFP-WPRE

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pIS50 KIF2 3'UTR mut

Plasmid

#14494

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

KIF2 3'UTR mutant (KIF2A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
SL535

Plasmid

#49941

Purpose

Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

Depositor

Keith Joung

Article

Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

Insert

Tet1CD RH-2 (RHOXF2 Human)

Use

TALEN

Promoter

EF1a

Available Since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only
mTFP1-Gamma-Tubulin-17

Plasmid

#55485

Purpose

Localization: Centrosomes, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Tubulin-6

Plasmid

#55097

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only