We narrowed to 59,446 results for: cloning

1. pC0.167

   Plasmid

   #119636

   Purpose

   Level 0 Part. Insertion site

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   6803 NS2 Up Flank
   

   Use

   Synthetic Biology

   Mutation

   non functional/large non-specific insertion

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pC0.168

   Plasmid

   #119635

   Purpose

   Level 0 Part. Insertion site

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   6803 NS2 Down Flank
   

   Use

   Synthetic Biology

   Mutation

   bp 630 A to T

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEN-R2-mCsfGFP-L3

   Plasmid

   #178602

   Purpose

   It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

   Depositor

   Frederica Theodoulou

   Article

   Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

   Insert

   Tandem fluorescent protein timers (tFTs)
   

   Use

   Entry vector for gateway cloning

   Tags

   mCherry superfolder GFP

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAT.011

   Plasmid

   #119555

   Purpose

   Self replication Level T acceptor vector with LacZ compatible blue/white screening. Modified SEVA vector, RK2 ori vector.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BB3aZ_14*

   Plasmid

   #98542

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Brigitte Gasser, Diethard Mattanovich, Michael Sauer

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCA0.002

   Plasmid

   #119560

   Purpose

   Level 0 acceptor vector for promoters with lacZ compatible with blue/white screening, low copy number, pSC101 ori.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPT-PCR C2

   Plasmid

   #141247

   Purpose

   PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Algal Research, 2020. 50, 102014

   Insert

   Mitochondrial Genome of Phaeodactylum tricornutum
   

   Use

   Synthetic Biology; Diatom expression

   Expression

   Bacterial and Yeast

   Mutation

   Repeat region removed, additional minor mutations…

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTL-Bag2T-gRNA2

   Plasmid

   #196253

   Purpose

   Plasmid for cloning the second CRISPR-Cas9 guide RNA for guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Expression

   Bacterial

   Promoter

   J23119 promoter

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Pmyo3::tag:sl1(G16C)

   Plasmid

   #79003

   Purpose

   Expression of exogenous SL1 in C. elegans muscle

   Depositor

   Xiao Liu, Ge Zhan

   Article

   Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

   Insert

   sls-1.2 (sls-1.2 Nematode)
   

   Tags

   illumina-adapter

   Expression

   Worm

   Mutation

   G16C

   Promoter

   myo-3

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCheck2 Dest (R1-R2) #BGV129

    Plasmid

    #48955

    Purpose

    Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Luciferase; Gateway cloning vector

    Promoter

    SV40

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

11. bdSUMO-R9-GFP-HiBit

    Plasmid

    #170990

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-R9-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDT-sgRNA-XMAS-2x

    Plasmid

    #164414

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-2xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZA1

    Plasmid

    #158479

    Purpose

    Entry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1 (ZAR1 Mustard Weed)
    

    Use

    Golden gate entry vector

    Mutation

    STOP codon

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Sha2Cas9

    Plasmid

    #192140

    Purpose

    Expresses Sha2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    Sha2Cas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SpeCas9

    Plasmid

    #192139

    Purpose

    Expresses SpeCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

    Insert

    SpeCas9
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFOSGE_DUM1H01

    Plasmid

    #71090

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    nej (nej Fly)
    

    Use

    Gateway entry vector

    Available Since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pQTEV-SARA1

    Plasmid

    #34809

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    SARA1 (SAR1A Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BB3aN_14*

    Plasmid

    #98548

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Brigitte Gasser, Diethard Mattanovich, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO V5 DNAJB5

    Plasmid

    #19527

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 5 (DNAJB5 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. E2_pENTR_R4R3_T CTP

    Plasmid

    #171736

    Purpose

    ptpTALECD vector assembly, Entry clone 2 with attR4 and R3

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    A terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge