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Plasmid#180226PurposeBaculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)DepositorInsertHistone deacetylase 6 (hdac6 Zebrafish)
TagsTwinStrep-3CExpressionInsectPromoterpolyhedrinAvailable sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEX301-RFP-GGA3 NGAT
Plasmid#162031PurposeExpresses NGAT sequence of GGA3DepositorInsertGGA3 NGAT (GGA3 Human)
UseLentiviralAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-TALE-mCherry-15b
Plasmid#138287PurposeExpress tTALE-mCherryDepositorInsert7-mer tTALE-mCherry
UseTALENTags6x His-tagExpressionBacterialPromoterT7 promoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-LRRK2 WD40
Plasmid#139211PurposeHuman LRRK2 WD40 domain (residues 2142–2527) was expressed in Sf9 insect cells via baculovirus infection and purified to homogeneity.DepositorAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-HMGS
Plasmid#108945Purposeheterologous expression of HMGS of E. faecalis in E. coliDepositorInsertHMG-CoA synthase (HMGS)
TagsHis6-TagExpressionBacterialMutationA110GAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-MVK mazei
Plasmid#108947Purposeheterologous expression of MVK of M. mazei in E. coliDepositorInsertMevalonate kinase (MVK)
TagsHis6-TagExpressionBacterialAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
POM43-mCitrine
Plasmid#69855PurposeN-terminal tagging with mCitrine using LEU2 as a selection marker in S. cerevisiae.DepositorInsertmCitrine minus start and stop codon
ExpressionYeastAvailable sinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRY2(delta2-6)-mCherry
Plasmid#104182PurposeExpresses fusion of CRY2(delta aa 2-6) with mCherryDepositorInsertCRY2(delta2-6)-mCherry
ExpressionMammalianMutationCRY2PHR with deletion of aa 2-6PromoterCMVAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag-RagC(L91P)
Plasmid#99726PurposeMammalian expression of Flag-RagC(L91P)DepositorAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Flag-RagC(K84T)
Plasmid#99727PurposeMammalian expression of Flag-RagC(K84T)DepositorAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.17.1
Plasmid#98189PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.23.1
Plasmid#98206PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.21.2
Plasmid#98200PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable sinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-005
Plasmid#78692PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorPublicationInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pISH-Neurod1
Plasmid#74396PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Neurod1. Not for gene expression.DepositorInsertneurogenic differentiation 1 (Neurod1 Mouse)
Available sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23100_AB
Plasmid#65980PurposeMoClo Basic Part: Constitutive promoter - Anderson series - high strength [A:J23100:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
peZinCh-1
Plasmid#24172DepositorInserteZinCh-1
ExpressionMammalianMutationCerulean and Citrine both contain mutations Y39H …PromoterCMVAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS SK mCherryROSAbsr
Plasmid#54321PurposeEncodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.DepositorInsertmCherry and BlasticidinS-resistance
UseCRISPR, Mouse Targeting, and TALENExpressionMammalianPromoterCMVAvailable sinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBUN250
Plasmid#60672Purposeoverexpression of M. smegmatis ddl gene in MycobacteriumDepositorInsertddl
UseE.coli-mycobacterium shuttle vectorPromoterBCG Hsp60Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA2_NANOG
Plasmid#64154PurposePhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only