Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 13,843 results for: 109

Showing: 7541 - 7560 of 13843 results

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pEPQD0CM0539

Plasmid

#162306

Purpose

Level 0 CDS part for MoClo assembly, AATG-TTCG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

CDS, TEV protease (S219V), no stop codon

Use

Synthetic Biology

Mutation

S219V, codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPMY0SP0002

Plasmid

#162304

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, 6xHis tag-[HRV 3C cleavage site]

Use

Synthetic Biology

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0551

Plasmid

#162303

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]

Use

Synthetic Biology

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0547

Plasmid

#162298

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, TrxA-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0546

Plasmid

#162297

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, MBP-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0544

Plasmid

#162295

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, MBP-[Factor Xa cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0543

Plasmid

#162294

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, GST-[TEV cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0541

Plasmid

#162292

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

N-terminal tag, GST-[thrombin cleavage site]

Use

Synthetic Biology

Mutation

codon optimized for E. coli

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0122

Plasmid

#154633

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

CaMV35S (C-CRE relocated) Firefly luciferase expression cassette [CaMV35S (C-CRE relocated) ::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0127

Plasmid

#154634

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

CaMV35S (C-CRE relocated) Firefly luciferase expression cassette [CaMV35S (C-CRE relocated) ::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0153

Plasmid

#154637

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MMV (C-CRE relocated) Firefly luciferase expression cassette [MMV (C-CRE relocated)::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0157

Plasmid

#154638

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

NOS (C-CRE relocated) Firefly luciferase expression cassette [NOS (C-CRE relocated)::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0158

Plasmid

#154639

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

NOS (C-CRE relocated) Firefly luciferase expression cassette [NOS (C-CRE relocated)::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0137

Plasmid

#154636

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MMV (C-CRE relocated) Firefly luciferase expression cassette [MMV (C-CRE relocated)::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr821

Plasmid

#136721

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr821

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr821 (Olfr821 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pFD-AI(TVMV)

Plasmid

#137111

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(TVMV)

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pXf20pemIK-GW

Plasmid

#126576

Purpose

Vector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability, Gateway cloning compatible

Depositor

Lindsey Burbank, Drake Stenger

Article

Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

Type

Empty backbone

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMXLP_eFq

Plasmid

#114248

Purpose

POC12390, MetClo assembly vector with p15A replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type e-q

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXBC_pFa

Plasmid

#114249

Purpose

POC12391, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type p-a

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXBC_aFb

Plasmid

#114250

Purpose

POC12392, MetClo assembly vector with F replication origin and chloramphenicol resistance for BsaI-based MetClo, adaptor sequence type a-b

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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