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pDMJ2-SARS-CoV-2-Spike (B.1-4d)

Plasmid

#241043

Purpose

Expresses SARS-CoV-2 spike (B.1-4d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76I,M153S,Y473F,Q493R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2c)

Plasmid

#241040

Purpose

Expresses SARS-CoV-2 spike (B.1-2c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,K417V,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2d)

Plasmid

#241041

Purpose

Expresses SARS-CoV-2 spike (B.1-2d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,K417V,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2a)

Plasmid

#241038

Purpose

Expresses SARS-CoV-2 spike (B.1-2a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T478K,R346T,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2b)

Plasmid

#241039

Purpose

Expresses SARS-CoV-2 spike (B.1-2b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,S494R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1)

Plasmid

#241033

Purpose

Expresses SARS-CoV-2 spike (B.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a-EGFP-hs-miR-126 (JDW 1415)

Plasmid

#242581

Purpose

An EF1a driven expression vector containing an EGFP fused to the human microRNA 126.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EGFP with an intron containing hs-miR-126 (MIR126 Human)

Expression

Mammalian

Promoter

human EF1a

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-NLSx3-miRFP703-dSal-p53NT(1-97)-L22Q/W23S/W53Q/F54S-lin-hCRY2

Plasmid

#241847

Purpose

Expresses a negative control of the actuator of Opto-p53 in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Tsuruoka et al Cell Struct Funct. 2025 Jun 12. doi: 10.

Insert

p53 (TP53 Human)

Tags

CRY2 and miRFP703

Expression

Mammalian

Mutation

N-terminus (1-97 aa) containing L22Q/W23S/W53Q/F5…

Promoter

CAGGS promoter

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-PDE6B-146

Plasmid

#239098

Purpose

pGL3 luciferase vector with 146bp PDE6B promoter (containing NRL response element).

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Insert

PDE6B promoter (PDE6B Human)

Use

Luciferase

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-SUMO-h-cGAS(T321I)

Plasmid

#232294

Purpose

Expression of mutant human cGAS protein (T321I)

Depositor

Thomas Tuschl

Article

Skeldon et al Commun Chem. 2025 Mar 22;8(1):88. doi: 1

Insert

MB21D1 (CGAS )

Mutation

T321I

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVenh synapsin-intron-mCherry-1x miR5 Mm ATP10B

Plasmid

#216391

Purpose

transfer plasmid for AAV vector production of mCherry and miR for Mm ATP10B

Depositor

Veerle Baekelandt

Article

Sanchiz-Calvo et al Acta Neuropathol. 2025 Jul 17;150(1):5.

Insert

mcherry and miR Mm ATP10B

Use

AAV

Promoter

CMVenh synapsin

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVenh synapsin-intron-mCherry-1x miR7 Rn ATP10B

Plasmid

#216392

Purpose

transfer plasmid for AAV vector production of mCherry and miR for rat Rn ATP10B

Depositor

Veerle Baekelandt

Article

Sanchiz-Calvo et al Acta Neuropathol. 2025 Jul 17;150(1):5.

Insert

mcherry and miR Rn ATP10B

Use

AAV

Promoter

CMVenh synapsin

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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sgYAP-R-1

Plasmid

#229445

Purpose

knockout of YAP1

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgYAP-R-1 (YAP1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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AAV 9P31-hSyn-Venus-P2A-Tau-RING I18R/M72E

Plasmid

#233694

Purpose

AAV expression of Venus-P2A-Tau-RING-I18R/M72E from hSyn promoter

Depositor

Leo James

Article

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Venus-P2A-Tau-RING I18R-M72E

Use

AAV

Expression

Mammalian

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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p5E-Dll4-F2-E1B-B-Globin (JDW 1366)

Plasmid

#229843

Purpose

A gateway compatible 5' entry clone containing the murine Dll4 F2 arterial specific enhancer upstream of a minimal E1b promoter and intron for arterial and endocardial expression.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Dll4-F2 (exon3)

Use

Gateway entry clone

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

Plasmid

#229811

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA transactivator-LexA-Operon-nls-GFP

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

Plasmid

#229813

Purpose

A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexAOp-cFos

Use

Tol2 based expression vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)

Plasmid

#229817

Purpose

A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

Plasmid

#229819

Purpose

A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Tol2 destination vector

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-DEST (JDW 1186)

Plasmid

#229820

Purpose

An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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