Addgene

We narrowed to 21,949 results for: ESC

Showing: 1481 - 1520 of 21949 results

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pReceiver

Plasmid

#228594

Purpose

Mammalian expression of a single fluorescent protein, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMulti

Plasmid

#228613

Purpose

Mammalian expression of a single fluorescent protein and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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CUP1-∆ssCPY*-mGFP

Plasmid

#212197

Purpose

Fluorescent reporter for cytoplasmic aggregates

Depositor

Mark Leake, Chris MacDonald

Insert

Carboxypeptidase Y variant (PRC1 Budding Yeast)

Use

Tags

mEGFP (monomeric version EGFP, A206K mutation)

Expression

Yeast

Mutation

Deletion signal peptide, G255A mutation

Promoter

CUP1

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pJRK259

Plasmid

#173752

Purpose

fluorescent par-6(delPB1) expression

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::mKate2:: par-6(_PB1)::unc-54 3' UTR

Use

Tags

Expression

Worm

Mutation

delPB1

Promoter

Prps-0

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_mTFP1-mVenus

Plasmid

#118859

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

mTFP1-mVenus

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 xenopus twisted gastrulation

Plasmid

#24680

Depositor

Edward De Robertis

Article

Oelgeschlager et al Nature. 2000 Jun 15. 405(6788):757-63.

Insert

twisted gastrulation (twsg1 Frog)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pSKB3.EG10680

Plasmid

#11436

Depositor

Sung-Hou Kim

Article

Shin et al Acta Crystallogr D Biol Crystallogr. 200

Insert

OsmC (osmC E. coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pMSCVpuro-Venus

Plasmid

#96940

Purpose

Retroviral plasmid containing Venus for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Venus Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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GW1-PercevalHR

Plasmid

#49082

Purpose

Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pACYC-GroEL/ES-TF

Plasmid

#83923

Purpose

Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Gro EL/ES
Trigger Factor

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBA2219

Plasmid

#38152

Depositor

David Waugh

Article

Austin et al Protein Expr Purif. 2012 Mar;82(1):116-2

Insert

DsbC (dsbC E. coli)

Use

Tags

N-terminal leader peptide (cloning artifact)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pPAmCherry-a-tubulin

Plasmid

#31930

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Use

Tags

a-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pRsetB-PercevalHR

Plasmid

#49081

Purpose

Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Article

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Use

Tags

7xHis

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pMBIS

Plasmid

#17817

Depositor

Jay Keasling

Article

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Insert

mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only

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SuperNova Green/ pRSETB

Plasmid

#124839

Purpose

bacterial expression of SuperNovaGreen, a green monomeric photosensitizing fluorescent protein

Depositor

Takeharu Nagai

Article

Riani et al BMC Biol. 2018 Apr 30;16(1):50. doi: 10.

Insert

SuperNova Green

Use

Tags

No tag

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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HRE-dUnaG

Plasmid

#201711

Purpose

Hypoxia responsive promoter driving destablized UnaG fluorescent protein

Depositor

Friedemann Kiefer

Article

Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

Insert

UnaG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDS1928

Plasmid

#167284

Purpose

Expression plasmid for Candida albicans containing a red-shifted luciferase

Depositor

Dominique Sanglard

Article

Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

Inserts

red shifted luciferase
SAT1

Use

Tags

Expression

Mutation

Promoter

ACT1

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_mCherry_mito Grx1-roGFP2

Plasmid

#155044

Purpose

Fluorescent redox reporter

Depositor

Vanessa LaPointe

Insert

Glutaredoxin-1 (GLRX Human)

Use

Lentiviral

Tags

mitochondrial targeting sequence and roGFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET-2(KW)2-mCherry

Plasmid

#112738

Purpose

Express 2(KW)2-mCherry in E. coli

Depositor

Kyubong Jo

Article

Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

Insert

2(KW)2-mCherry

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-CalfluxVTN

Plasmid

#83926

Purpose

Bioluminescent Reporter of Intracellular Calcium

Depositor

Carl Johnson

Article

Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

Insert

CalfluxVTN

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-tTA

Plasmid

#104109

Purpose

AAV-mediated expression of tTA under the Syn1 promoter.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

tTA2

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pKSI-1

Plasmid

#51725

Purpose

cloning backbone

Depositor

Sheng Yang

Article

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

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prsTagRFP-C1

Plasmid

#31926

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Use

Tags

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-N1

Plasmid

#79988

Purpose

Monomeric near-infrared fluorescent protein miRFP703 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP703

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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Sirius/pRSETB

Plasmid

#51956

Purpose

bacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

Depositor

Takeharu Nagai

Article

Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

Insert

Sirius

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pRSetB-Peredox

Plasmid

#73806

Purpose

Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tag

Depositor

Gary Yellen

Article

Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Insert

Peredox

Use

Tags

his-tag to facilitate purification

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-TF

Plasmid

#83921

Purpose

Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Trigger Factor

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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Ec2Avs2

Plasmid

#188790

Purpose

Avs2 from Escherichia coli NCTC10650

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

Ec2Avs2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Skylan-S-N1

Plasmid

#129737

Purpose

A green photo-switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI)

Depositor

Pingyong Xu

Article

Zhang et al ACS Nano. 2015 Mar 24;9(3):2659-67. doi:

Type

Empty backbone

Use

Tags

Skylan-S

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pmiRFP709-N1

Plasmid

#79989

Purpose

Monomeric near-infrared fluorescent protein miRFP709 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP709

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.1(-) VSFP3.1

Plasmid

#18951

Depositor

Thomas Knopfel

Article

Lundby et al PLoS ONE. 2008 . 3(6):e2514.

Insert

VSFP3.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCVpuro-mIFP

Plasmid

#96951

Purpose

Retroviral plasmid containing mIFP for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Infrared Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIS.MTRIA-OT

Plasmid

#184596

Purpose

For expression of a fluorescent sensor for oxytocin in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-OT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCas1+2

Plasmid

#72676

Purpose

Express Cas1 and Cas2 for the CRISPR adaptation process in Escherichia coli. Has streptomycin resistance.

Depositor

Udi Qimron

Article

Yosef et al Nucleic Acids Res. 2012 Jul;40(12):5569-

Insert

Cas1, Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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csr-1_hH1_mEos3.1_V5

Plasmid

#191750

Purpose

Photoconvertible fluorescent nuclear reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

hH1 (hH1 Neurospora crassa)

Use

Tags

V5, ccg-1 promoter, and mEos3.1

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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vgCam-pRSETb

Plasmid

#193362

Purpose

A FRET-based calcium probe using Sumire

Depositor

Takeharu Nagai

Article

Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

Insert

vgCam

Use

E.coli expression

Tags

His X6 tag and Strep tag

Expression

Mutation

Promoter

T7

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET22-HT/spoR

Plasmid

#73631

Purpose

Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Depositor

James Williamson, T. J. Kappock

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

guanylate kinase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-TRE-mTurquoise2-WPRE

Plasmid

#104103

Purpose

Expresses mTurquoise2 under the TRE promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Sakaguchi et al eLife. 2018 Nov 20;7. pii: 40350. doi: 1

Insert

mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-T7-SdB-T

Plasmid

#87307

Purpose

Expresses the fluorescence-activating Stabilized dimeric Broccoli (SdB) aptamer under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Stabilized dimeric Broccoli aptamer

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EcAvs4

Plasmid

#188794

Purpose

Avs4 from Escherichia coli NCTC11132

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

EcAvs4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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