Addgene

We narrowed to 17,834 results for: REV

Showing: 1851 - 1900 of 17834 results

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pK3F

Plasmid

#85770

Purpose

N-ICE plasmid pK3F

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pXZX352

Plasmid

#169456

Purpose

MATA fragment in pTOPO vector

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

Mata locus

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pXZX353

Plasmid

#169457

Purpose

MATα (alpha) fragment in pTOPO vector

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

MatA

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pK3F-GAL1

Plasmid

#85772

Purpose

N-ICE plasmid pK3F-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pH3FS-PYK1

Plasmid

#85781

Purpose

N-ICE plasmid pH3FS-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pL-rpb1-N488D

Plasmid

#10988

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-N488D (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 1462 A to G in the RPB1 ORF resulting in…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pK3FS-CYC1

Plasmid

#85779

Purpose

N-ICE plasmid pK3FS-CYC1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-CYC1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJW1510

Plasmid

#62364

Purpose

pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

BirA-mVenus-Ubc6 tail anchor

Use

Tags

Expression

Yeast

Mutation

L232H mutation compared to standard mVenus

Promoter

Sec63

Available since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-GST-TRP1

Plasmid

#41603

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GST and yeast ADH1 terminator

Expression

Mutation

Promoter

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pK3FS-GAL1

Plasmid

#85778

Purpose

N-ICE plasmid pK3FS-GAL1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-P30-His-POL30-3'

Plasmid

#99546

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

POL30

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p416TEF-SEC16

Plasmid

#102676

Purpose

The gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretion

Depositor

Jens Nielsen

Article

Bao et al Appl Environ Microbiol. 2017 Jun 30;83(1

Insert

SEC16 (SEC16 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pTCW008

Plasmid

#83526

Purpose

pheromone mediated alpha pheromone expression in yeast from the mfalpha 2 gene and FUSJ2 promoter

Depositor

Claudia Vickers

Article

Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

Insert

pFUS1J2-mfalpha2

Use

Tags

Expression

Yeast

Mutation

Promoter

pFUS1J2

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pTEF-Sup35C

Plasmid

#1202

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35 C-terminus (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Sup35 C-terminus ~254aa-end

Promoter

TEF

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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pJC25SUP35

Plasmid

#1096

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pH3F

Plasmid

#85773

Purpose

N-ICE plasmid pH3F

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pNMG

Plasmid

#1203

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

NM region of Sup35 (SUP35 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

NM region (1-253aa) of Sup35

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-P30-His-pol30(K164R)-3'

Plasmid

#99548

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K164R

Promoter

POL30

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM2_4

Plasmid

#183057

Purpose

HAA1-mTurquoise2 under HAA1p and mRuby2 under YGP1p with flanks for genome integration into HO locus

Depositor

Yvonne Nygård

Article

Mormino et al FEMS Yeast Res. 2021 Sep 11;21(6). pii:

Inserts

mRuby2
HAA1-mTurquoise2

Use

Synthetic Biology

Tags

mRuby2 and mTurquoise2

Expression

Yeast

Mutation

Promoter

HAA1 and YGP1

Available since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-CDC12(WT)

Plasmid

#1168

Depositor

Susan Lindquist

Article

Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

Insert

CDC12 (CDC12 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Added N-terminal GFP to CDC12 in plasmid CDC12(WT…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-Num1-PH

Plasmid

#71374

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Num1-PH

Use

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa2563-2692

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pMM0623

Plasmid

#128998

Purpose

pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and ConR2

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-ConR2 AmpR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0624

Plasmid

#128999

Purpose

pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0621

Plasmid

#128996

Purpose

pRPL18B-VP16-CIB1 cassette plasmid flanked by ConLS and ConR1

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConLS_pRPL18B-SV40NLS-VP16-CIB1-tENO1-ConR1 AmpR Col-E1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZ414-F55

Plasmid

#23079

Depositor

Sharon Dent

Article

Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

Insert

yeast Histone H3-2 and Histone H4-2

Use

Tags

Expression

Yeast

Mutation

Histone H3 changed Lysine 9 to Arginine, H3 K9R …

Promoter

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pMM0617

Plasmid

#128993

Purpose

loxP-KIURA3-loxP preassembled entry vector

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

KanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPS1601

Plasmid

#8921

Depositor

Pamela Silver

Article

Valentini et al Genetics. 2002 Feb . 160(2):393-405.

Insert

PAB1 (PAB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
YIp204-P30-His-POL30

Plasmid

#99550

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); TRP1 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

POL30

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCuHA-ATG13[3SA](426)

Plasmid

#122076

Purpose

Expressing HA-ATG13[3SA]

Depositor

Daniel Klionsky

Article

Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

Insert

ATG13

Use

Tags

3HA

Expression

Yeast

Mutation

Serine 646 to alanine; Serine 649 to alanine; Ser…

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
piHGpd/p82

Plasmid

#1105

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

wild-type HSP82 regulated by the strong constitut…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/G170D

Plasmid

#1113

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with glycine 170 changed to aspartic acid; …

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pK3F-PYK1

Plasmid

#85771

Purpose

N-ICE plasmid pK3F-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pL-rpb1-C67Y

Plasmid

#10982

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-C67Y (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 200 G to A in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-C70Y

Plasmid

#10983

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-C70Y (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 209 G to A in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-H80Y

Plasmid

#10984

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-H80Y (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 238 C to T in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
sup35-C653R

Plasmid

#1198

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35p (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

changed cys 653 to arg

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pTG005

Plasmid

#71386

Purpose

localization of yeast RNase RMP1 (RNase MRP binding protein) in yeast

Depositor

Mark Schmitt

Article

Gill et al J Cell Biol. 2006 Apr 10;173(1):35-45. E

Insert

RMP1 (RMP1 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

ACT1

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-P30-His-pol30(K127R)-3'

Plasmid

#99547

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K127R

Promoter

POL30

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
sup35-R320I

Plasmid

#1199

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35p (SUP35 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

changed arg 320 to isoleucine

Promoter

Available since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
pGLx2-set1deltaRRM

Plasmid

#53257

Purpose

GAL driven LexA-tagged SET1-deltaRRM (RRM domain deleted)

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Use

Tags

LexA

Expression

Yeast

Mutation

RRM domain deleted (aa 230-355)

Promoter

GAL1/10

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pPS1597

Plasmid

#8920

Depositor

Pamela Silver

Article

Valentini et al Genetics. 2002 Feb . 160(2):393-405.

Insert

TIF51A (HYP2 Budding Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/T101I

Plasmid

#1112

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with threonine 101 changed to isoleucine; r…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-D260N

Plasmid

#10985

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D260N (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 778 G to A in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-D261N

Plasmid

#10986

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-D261N (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 781 G to A in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-R320C

Plasmid

#10987

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-R320C (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 958 C to T in the RPB1 ORF resulting i…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-E1230K

Plasmid

#10990

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-E1230K (RPO21 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Mutation 3688 G to A in the RPB1 ORF resulting in…

Promoter

Available since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pYO411

Plasmid

#235732

Purpose

Protein expression of ZZ-FL yeast Atg14 in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ATG14

Use

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO831

Plasmid

#235754

Purpose

Protein expression of ZZ-ScVPS38 (1-212F)

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Use

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 1-212F

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO225

Plasmid

#235729

Purpose

Protin expression of FL yeast VPS15 -ZZ in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS15

Use

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO290

Plasmid

#235731

Purpose

Protein expression in FL yeast VPS34 D731N mutant in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Use

Tags

Expression

Yeast

Mutation

S2A, D731N

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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