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pJC20Tpm1

Plasmid

#99355

Purpose

Bacterial expression of budding yeast (S. cerevisiae) Tropomyosin, Tpm1

Depositor

Dan Mulvihill

Article

Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

Insert

Tropomyosin (TPM1 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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fSAP24-GST

Plasmid

#11202

Depositor

Robin Reed

Article

Masuda et al Genes Dev. 2005 Jul 1. 19(13):1512-7.

Insert

SAP24 (THOC7 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
pJC20cdc8

Plasmid

#99354

Purpose

Bacterial expression of fission yeast (S. pombe) Tropomyosin, cdc8

Depositor

Dan Mulvihill

Article

Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60

Plasmid

#49090

Purpose

Template for generating full length gene 60 mRNA by in vitro transcription with T7 RNA pol

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Promoter

T7

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCEF-FKBP12-Bla2

Plasmid

#190946

Purpose

Protein fragment complementation

Depositor

Jakob Reiser

Article

Ou et al Gene Ther. 2013 Jan;20(1):43-50. doi: 10

Insert

C-terminal fragment of BLAK fused to FKBP12

Expression

Mammalian

Promoter

CEF

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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CAA 659

Plasmid

#108684

Purpose

in vitro mRNA transcription

Depositor

Jon Lorsch

Article

Yourik et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

CAA repeat mRNA

Expression

Bacterial

Promoter

T7

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJC20Tpm2

Plasmid

#99356

Purpose

Bacterial expression of budding yeast (S. cerevisiae) Tropomyosin, Tpm2

Depositor

Dan Mulvihill

Article

Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

Insert

Tropomyosin (TPM2 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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SJZ9-amdS

Plasmid

#228261

Purpose

Template plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen source

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

amdS

Expression

Yeast

Promoter

ENO2 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ5-HPH

Plasmid

#228258

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

hph

Expression

Yeast

Promoter

SSA1 S.c.

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ7-KAN

Plasmid

#228260

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

kan

Expression

Yeast

Promoter

GPM1 S.c.

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ4-NAT

Plasmid

#228257

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

nat1

Expression

Yeast

Promoter

FBA1 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCAMBIA-FLS2prom::FLS2-3xmyc

Plasmid

#86158

Purpose

FLS2 expression

Depositor

Silke Robatzek

Article

Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

Insert

FLS2 (FLS2 Mustard Weed)

Tags

3xmyc

Expression

Plant

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD33(A-N)

Plasmid

#187084

Purpose

Medium expression of Complex I with subunit N-HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Tags

HA tag nuoN

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCR-Blunt_hM3O

Plasmid

#112638

Purpose

Plasmid for PCR amplification of hM3O DNA template for in vitro transcription

Depositor

Ganna Bilousova

Article

McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

Insert

hM3O

Use

Pcr template

Promoter

T7

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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MKT074

Plasmid

#231997

Purpose

MicF RNA toehold switch with NanoLuc complementation peptide reporter

Depositor

Melissa Takahashi

Article

Diaz et al MicroPubl Biol. 2025 Jan 2;2025:10.17912

Insert

MicF RNA toehold switch

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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SJZ2-TRP1

Plasmid

#228256

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker TRP1 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

TRP1

Expression

Yeast

Promoter

BBC1 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ10-LEU2

Plasmid

#228262

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker LEU2 (Kluyveromyces lactis)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

LEU2

Expression

Yeast

Promoter

LEU2 K.l.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD33(A-H*HA-N)

Plasmid

#187086

Purpose

Medium expression of Complex I with subunits N and H -HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Tags

HA tags nuoN, nuoH

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCR-Blunt_mWasabi

Plasmid

#112640

Purpose

Plasmid for PCR amplification of mWasabi DNA template for in vitro transcription

Depositor

Ganna Bilousova

Article

McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

Insert

mWasabi

Use

Pcr template

Promoter

T7

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pILGFP3AA5

Plasmid

#185875

Purpose

A plasmid for in vivo gene amplification (HapAmp) at RPL25 locus (~47 copy)

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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