We narrowed to 21,803 results for: SPR

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pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

Plasmid

#175571

Purpose

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)

Plasmid

#185917

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8.20m-nSpRY-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)

Plasmid

#175573

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

Plasmid

#175572

Purpose

Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

Plasmid

#138146

Purpose

Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NES

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi)

Plasmid

#175574

Purpose

Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression

Depositor

Joseph Brzezinski

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE

Plasmid

#138145

Purpose

Expresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

PspCas13b

Use

Lentiviral

Tags

NLS

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-CASPR/Neurexin IV [K65/35R] (Recombinant Antibody)

Antibody

#182999-rAb

Purpose

Anti-CASPR/Neurexin IV (Rat) recombinant mouse monoclonal antibody

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Recommended Applications

Immunohistochemistry and Western Blot

Reactivity

Human, Mouse, and Rat

Source Species

Mouse

Isotype

IgG2a

Trial Size

Available to purchase

Available Since

May 31, 2022

Availability

Industry, Academic Institutions, and Nonprofits
lentiCRISPRv2_ISG

Pooled Library

#125753

Purpose

Knockout library targeting a set of human interferon-stimulated genes (ISGs).

Depositor

Michael Emerman, Molly OhAinle

Article

Roesch et al Retrovirology. 2018 Mar 20;15(1):26. doi

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only
The Human Druggable CRISPR library

Pooled Library

#200013

Purpose

Knockout library containing ~10,000 sgRNAs targeting 1,980 human druggable genes (5 sgRNAs per gene and 100 non-targeting control sgRNAs).

Depositor

Lianxin Liu

Article

Zheng et al Adv Sci (Weinh). 2023 Aug;10(22):e220679

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only
Human CRISPR lncRNA activation library (SAM) in lenti sgRNA(MS2)_zeo backbone

Pooled Library

#1000000106

Purpose

Human CRISPR Synergistic Activation Mediator (SAM) pooled library for the transcriptional activation of lncRNAs

Depositor

Feng Zhang

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only
Mouse Brie kinome pooled library, guides 1-4 in lentiCRISPRv2 backbone

Pooled Library

#75317

Purpose

Mouse sgRNA library in backbone lentiCRISPRv2 targeting 713 kinase genes and containing 2,852 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pTn7-SCOUT14

Plasmid

#201015

Purpose

pLv1-pUC18R6KT-miniTn7-aad9, SpR, StR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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Human ubiquitination-related proteins CRISPR KO library

Pooled Library

#174592

Purpose

Targets human ubiquitation-related proteins and deubiquitinating enzymes.

Depositor

Juan Bonifacino

Article

Jia et al Elife. 2019 Nov 6;8. pii: 50034. doi: 10

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only
hCRISPRi-v2, Membrane Proteins (h6), top 5 sgRNAs/gene

Pooled Library

#83976

Purpose

Human CRISPRi Pooled Library targeting membrane proteins

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Membrane proteins

Pooled Library

#1000000124

Purpose

CRISPR gRNA mouse knockout pooled library - Membrane proteins

Depositor

Michael Bassik

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only
hCRISPRa-v2, Kinases, Phosphatases, and Drug Targets (h1), top 5 sgRNAs/gene

Pooled Library

#83980

Purpose

Human CRISPRa Pooled Library tartgeting kinsases, phosphatases and Drug Targets

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases

Pooled Library

#1000000122

Purpose

CRISPR gRNA mouse knockout pooled library - Drug targets, kinases, phosphatases

Depositor

Michael Bassik

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only
hCRISPRa-v2, Membrane Proteins (h6), top 5 sgRNAs/gene

Pooled Library

#83985

Purpose

Human CRISPRa Pooled Library targeting membrane proteins

Depositor

Jonathan Weissman

Article

Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only
LC-E75

Bacterial Strain

#115925

Purpose

Expression of dCas9 gene under the control of a weak Ptet promoter in the E. coli chromosome

Depositor

David Bikard

Article

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

Bacterial Resistance

None

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only