Purpose
sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.
Inserts
Toxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry
Expression
Bacterial
Mutation
Promoter
P3 constitutive promoter and native promoter
Available since
April 16, 2025
Availability
Academic Institutions and Nonprofits only