We narrowed to 5,561 results for: donor

1. pCRISPaint-T2A-sfGFP-3xP3-RFP

   Plasmid

   #127557

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sfGFP
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-T2A-Gal80-3xP3-RFP

   Plasmid

   #127562

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Gal80
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPaint-T2A-FLPo-3xP3-RFP

   Plasmid

   #127561

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   FLPo
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMH3

   Plasmid

   #52528

   Purpose

   serves as PCR-template to generate HR donors for C-terminal GFP-tag

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   eGFP and Blasticigin resistance cassette
   

   Use

   Blunt cloning kit

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPaint-T2A-Gal4DBD-3xP3-RFP

   Plasmid

   #127564

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Gal4DBD
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   July 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPaint-T2A-p65-3xP3-RFP

   Plasmid

   #127565

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   p65
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPaint-T2A-QF2-3xP3-RFP

   Plasmid

   #127559

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   QF2
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMH4

   Plasmid

   #52529

   Purpose

   serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   2x FLAG-tag
   

   Use

   Blunt cloning kit

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPaint-T2A-Nluc-3xP3-RFP

   Plasmid

   #127563

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Nluc
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDOC-K-glmS-mPlum

    Plasmid

    #158062

    Purpose

    Donor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    monomeric plum fluoresecent protein
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    acpP

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIW1

    Plasmid

    #52530

    Purpose

    serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Twin-STREP-tag
    

    Use

    Blunt cloning kit

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRB20

    Plasmid

    #52559

    Purpose

    serves as PCR-template to generate HR donors for C-terminal YFP-tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    eYFP and Blasticigin resistance cassette
    

    Use

    Blunt cloning kit

    Available Since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDOC-K-glmS-GFPrev

    Plasmid

    #158060

    Purpose

    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable marker

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    Green Fluorescent Protein optimised for excitation with UV light
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    acpP

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. ORFtag_TetR-SD_Frame1

    Plasmid

    #220981

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame1

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    PGK promoter

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pScarlessHD-sfGFP-DsRed

    Plasmid

    #80811

    Purpose

    Vector containing a cassette for scarless insertion of sfGFP and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    sfGFP-piggyBac-DsRed
    

    Use

    Unspecified

    Tags

    sfGFP

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

    Plasmid

    #175571

    Purpose

    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

    Plasmid

    #175572

    Purpose

    Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid

    #226429

    Purpose

    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.

    Depositor

    Alon Goren

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pScarlessHD-3xFLAG-DsRed

    Plasmid

    #80820

    Purpose

    Vector containing a cassette for scarless insertion of 3xFLAG and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    3xFLAG-piggyBac-DsRed
    

    Use

    Unspecified

    Tags

    3xFLAG

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pScarlessHD-2xHA-DsRed

    Plasmid

    #80822

    Purpose

    Vector containing a cassette for scarless insertion of 2xHA and marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Kate O'Connor-Giles

    Insert

    2xHA-piggyBac-DsRed
    

    Use

    Unspecified

    Tags

    2xHA

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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