We narrowed to 9,647 results for: bli

1. pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald

   Plasmid

   #201186

   Purpose

   Lentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis

   Inserts

   PTPN2, transcript variant 1 (PTPN2 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pdCas9-NED

   Plasmid

   #109358

   Purpose

   Mammalian vector for expressing effector domains fused to dCas9.

   Depositor

   Antal Kiss

   Article

   Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   dCas9, SV40 NLS, 3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Mouse 3' Hp1a AID GFP PuroR

   Plasmid

   #127897

   Purpose

   HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene

   Depositor

   Mark Groudine

   Insert

   Hp1a (Cbx5 Mouse, Mustard Weed)
   

   Use

   Donor plasmid

   Tags

   GFP, AID, PuroR

   Mutation

   Inserting GFP AID 2A Puro into mouse 3' Hp1a

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mApple-hCOL1A1

   Plasmid

   #140113

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available Since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFP.R267

   Plasmid

   #138179

   Purpose

   Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(SGY).gp41-1.(SSS)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-UBC9-K14R-A129K

   Plasmid

   #228948

   Purpose

   express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Mutation

   changed Lysine 14 to Arginine, changed Alanine 12…

   Promoter

   T7-lac

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJBJZ_003

   Plasmid

   #155358

   Purpose

   Rifampicin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

   Depositor

   Elisabeth Raleigh

   Article

   Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   Sept. 30, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pFP.R377

   Plasmid

   #138247

   Purpose

   Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(HDG).Mja-KlbA*.(CSG)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. DT12A

   Plasmid

   #80416

   Purpose

   Expresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

   Depositor

   Thomas Cooper

   Article

   Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

   Insert

   human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mVenus-hCOL1A1

    Plasmid

    #140111

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQTEV-FLJ30046

    Plasmid

    #34828

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    FLJ30046 (SLAIN1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQTEV-SCP2

    Plasmid

    #31336

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    sterol carrier protein 2 (SCP2 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AMPKA2

    Plasmid

    #36194

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    AMPKA2 (PRKAA2 Human)
    

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Mutation

    contains KINASE DOMAIN only

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pGL4.10/RSV_XS3B-miniXon_Luciferase

    Plasmid

    #217302

    Purpose

    Plasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by L

    Depositor

    Beverly L. Davidson

    Insert

    XS3B-miniXon
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Kozak ATG initiation sequence in middle exon

    Promoter

    RSV

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Galt1-sCGrx1p-HA

    Plasmid

    #230028

    Purpose

    Expressing sCGrx1p protein fused to HA tag in mammalian cells with localization in the Golgi apparatus.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Galt1-sCGrx1p-HA (B4GALT1 Human, Synthetic)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRN3P_AID-Dam-only

    Plasmid

    #136065

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    AID-Dam only
    

    Use

    In vitro transcription

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQTEV-RPL9

    Plasmid

    #31334

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    ribosomal protein L9 (RPL9 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. B52_puro_empty_gRNA

    Plasmid

    #197557

    Purpose

    sites for cloning two gRNA with puromycin cassette

    Depositor

    Darius Bägli

    Type

    Empty backbone

    Use

    Empty grna backbone with puromycin resistance gene

    Expression

    Mammalian

    Promoter

    U6 for gRNA, CMV for puromycin resistance.

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RabV CVS-N2c(deltaG)-dsRed

    Plasmid

    #73460

    Purpose

    Expresses dsRed for monosynaptic circuit mapping

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    dsRed
    

    Use

    Neurotropic virus

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RabV CVS-N2c(deltaG)-Cre

    Plasmid

    #73474

    Purpose

    Expresses Cre for circuit mapping and genetic manipulation

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    Cre recombinase
    

    Use

    Neurotropic virus

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge