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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,732 results for: bli

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Showing: 7581 - 7600 of 9732 results

  1. pAAV-hSyn-DIO-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid
    #50455
    Purpose
    Double floxed Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promoter
    Depositor
    Bryan Roth
    Has Service
    AAV8
    Insert
    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    Use
    AAV
    Tags
    HA
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    human Synapsin 1
    Available Since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50455-plasmid-map-sequence-id-214301

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  3. pc DNA FRT TO- APP695

    Plasmid
    #114193
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Expression
    Mammalian
    Mutation
    wild type splice variant 695: 695AA (2085bp, lack…
    Promoter
    CMV
    Available Since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114193-plasmid-map-sequence-id-224265

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  5. pBHM2681

    Plasmid
    #241726
    Purpose
    Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains ptxD marker for selection on media containing phosphite as a sole phosphorus source
    Depositor
    Hiten Madhani
    Article
    Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Sept. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241726-plasmid-map-sequence-id-479856

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  6. pLenti Puro HA-Ubiquitin-WT

    Plasmid
    #236289
    Purpose
    Lentiviral vector expressing HA-tagged wild type ubiquitin
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Ubiquitin (UBB Human)
    Use
    Lentiviral
    Tags
    HA
    Mutation
    Single ubiquitin coding sequence (full length UBB…
    Promoter
    CMV
    Available Since
    April 10, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236289-plasmid-map-sequence-id-467836

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  7. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

    Plasmid
    #69546
    Purpose
    Tet-On system expressing MyoD-2A-GFP-2xNLS.
    Depositor
    Charles Gersbach
    Inserts
    eGFP
    Myod1 (Myod1 Mouse)
    Use
    Lentiviral
    Tags
    2x nuclear localization sequence and T2A "sk…
    Mutation
    Removed stop codon to fuse it to the 2A-eGFPnls i…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69546-plasmid-map-sequence-id-122992

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  8. 7MEQ

    Plasmid
    #176412
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
    Use
    Baculovirus expresssion
    Tags
    8 x His Tag and Honeybee melittin signal
    Mutation
    etmprss2_S251D/R252D/Q253D/S254D/R255K
    Promoter
    Polyhedrin
    Available Since
    Oct. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    176412-plasmid-map-sequence-id-345854

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  9. pFUSE-rabbitFc1-IL2ss-VHH(G08)-His-Myc

    Plasmid
    #209857
    Purpose
    Plasmid coding for a nanobody (termed G08) against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1
    Depositor
    Tim Brown
    Inserts
    IL2-ss
    VHH (G08 variant)
    Rabbit Fc Gamma 1
    Tags
    x3 MycTag and x6 HisTag
    Expression
    Mammalian
    Promoter
    hEF1-HTLV promoter
    Available Since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    209857-plasmid-map-sequence-id-414817

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  10. pDule-Tet3.0

    Plasmid
    #174080
    Purpose
    Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-
    Insert
    Tet3.0 tRNA synthetase and tRNA
    Expression
    Bacterial
    Mutation
    L270G, Y271L, N311G, C313A
    Available Since
    Jan. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    174080-plasmid-map-sequence-id-351340

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  12. B52_puro_empty_gRNA

    Plasmid
    #197557
    Purpose
    sites for cloning two gRNA with puromycin cassette
    Depositor
    Darius Bägli
    Type
    Empty backbone
    Use
    Empty grna backbone with puromycin resistance gene
    Expression
    Mammalian
    Promoter
    U6 for gRNA, CMV for puromycin resistance.
    Available Since
    March 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    197557-plasmid-map-sequence-id-388707

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  13. pFP.R270

    Plasmid
    #138219
    Purpose
    Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GGG).IMPDH-1.(SIC)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138219-plasmid-map-sequence-id-273501

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  14. HaloTag-LacI-mHP1a

    Plasmid
    #232637
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with effector protein mouse HP1alpha
    Depositor
    Karsten Rippe
    Insert
    HaloTag-LacI-mHP1a (Cbx5 Synthetic, Mouse)
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232637-plasmid-map-sequence-id-463625

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  15. pFP.R269

    Plasmid
    #138181
    Purpose
    Expresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(NPC).NrdJ-1.(SEI)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138181-plasmid-map-sequence-id-296586

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  19. PB_p300_core-dCas9-EGFP_hygro

    Plasmid
    #226431
    Purpose
    PiggyBac transposon vector containing a dox inducible p300 core-dCas9 fusion protein and EGFP reporter. Hygromycin selection marker
    Depositor
    Alon Goren
    Inserts
    p300 core-dCas9-EGFP fusion protein
    rtTA3-IRES-Hygro
    Use
    CRISPR and Synthetic Biology; Piggybac transposon
    Tags
    HA tag, P2A-EGFP, and p300 core
    Expression
    Mammalian
    Promoter
    Dox inducible minimal CMV and UbC Promoter
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226431-plasmid-map-sequence-id-458102

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  20. pFP.R268

    Plasmid
    #138180
    Purpose
    Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(LNR).gp41-8.(SAV)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138180-plasmid-map-sequence-id-273503

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Showing: 7581 - 7600 of 9732 results