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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,351 results for: multi

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Showing: 7621 - 7640 of 18351 results

  2. ACV

    Plasmid
    #27791
    Depositor
    Steven Vogel
    Publication
    Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Amber-Cerulean-Venus
    Use
    Stoichiometry standard
    Expression
    Mammalian
    Available since
    June 23, 2011
    Availability
    Academic Institutions and Nonprofits only

  4. enOgeuIscB with ωRNA-v13

    Plasmid
    #220940
    Purpose
    CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloing
    Depositor
    Weixin Tang
    Publication
    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/
    Inserts
    CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
    OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220940-plasmid-map-sequence-id-443125

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  5. pUASp-GBP-Tbro

    Plasmid
    #179995
    Purpose
    Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.
    Depositor
    Graydon Gonsalvez
    Publication
    Baker et al Development. 2021 Nov 15;148(22). pii: 2
    Insert
    GBP nanobody
    Tags
    Trbo
    Expression
    Insect
    Promoter
    UAS-Gal4
    Available since
    March 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    179995-plasmid-map-sequence-id-357336

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  8. pADH140

    Plasmid
    #90988
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Publication
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    C. maltosa LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    Use
    CRISPR
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90988-plasmid-map-sequence-id-180521

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  9. pBP-J23100

    Plasmid
    #72963
    Purpose
    Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)
    Depositor
    Paul Freemont
    Publication
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    J23100 promoter
    Use
    Synthetic Biology
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72963-plasmid-map-sequence-id-133808

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  12. pLSLGUS

    Plasmid
    #51517
    Purpose
    pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Publication
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    GUS
    3kb filler sequence
    Use
    Plant t-dna plasmid
    Tags
    NLS and flag
    Promoter
    virion-sense LIR and 2x35S
    Available since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51517-plasmid-map-sequence-id-78696

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  16. GPRC5D-DuET

    Plasmid
    #213298
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPRC5D (GPRC5D Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213298-plasmid-map-sequence-id-430842

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  19. pEVOL-AcKRS-CloDF

    Plasmid
    #127415
    Purpose
    Mutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophage
    Depositor
    Wenshe Liu
    Publication
    Wang et al Angew Chem Int Ed Engl. 2019 Oct 28;58(4
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Promoter
    araBAD
    Available since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    127415-plasmid-map-sequence-id-250212

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  20. evoCas9

    Plasmid
    #138558
    Purpose
    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    evoCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138558-plasmid-map-sequence-id-274774

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Showing: 7621 - 7640 of 18351 results