We narrowed to 42,057 results for: kan

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pSSRB274_pAC8-eGFP-hsCRBN

Plasmid

#218791

Purpose

Insect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBN

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

Protein cereblon (CRBN Human)

Tags

FLAG-TEV-eGFP-Prescission

Expression

Insect

Mutation

eGFP fused to N-terminus

Promoter

polyhedrin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5_mEGFP-SS18-SSX1

Plasmid

#237678

Purpose

For overexpression of mEGFP-SS18-SSX1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

SS18-SSX1 (SS18, SSX1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3CA-FLAG

Plasmid

#179134

Purpose

Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_Puro_Telo_v2

Plasmid

#195139

Purpose

~100 repeats of the human telomere seed sequence fused to the puromycin resistance gene; digest with BstZ17I-HF and KpnI-HF to obtain transfectable construct for chromosomal arm loss induction

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Telomere (RTEL1 Human)

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOne-Puro-MDM4

Plasmid

#195140

Purpose

Tet-ON 3rd generation expression vector with inducible expression of MDM4 and a puromycin selection cassette

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 (MDM4 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSDC803_IKZF1_mutant

Plasmid

#224113

Purpose

Insect cell expression vector for IKZF1 mutant for CRBN cryoEM pre-blotting

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

IKZF1 (IKZF1 Human)

Tags

Flag-TEV

Expression

Insect

Mutation

Residues 140-196, Q146A and G151N

Promoter

polyhedrin

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

Plasmid

#216250

Purpose

HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

HDR template for human SEC61B (SEC61B Synthetic, Human, HDR template)

Use

CRISPR; Hdr template

Tags

BSD.P2A.miniIAA7.3xFlag

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET-45b-POLR2A-CTD-mCherry

Plasmid

#185011

Purpose

Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

POLR2A (Polr2a Mouse)

Expression

Bacterial

Available Since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA

Plasmid

#113160

Purpose

A plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxP

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-MEKDD

Plasmid

#31202

Purpose

Gateway entry vector containing MEKDD.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

MAPKK1 (MAP2K1 Human)

Use

Gateway donor vector

Mutation

S218D S222D

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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pCR4_miniFXN_7

Plasmid

#234360

Purpose

Mini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.

Depositor

Marek Napierala

Article

Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85

Insert

FXN (FXN Human)

Use

Cloning

Tags

Flag Tag

Mutation

There is a partial deletion in the 5'UTR of …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-TBP(38Q)-IDR-mCherry-Cry2-NLS

Plasmid

#145269

Purpose

Mammalian Expression of Nuclear Opto TBP(38Q) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBP(38Q)-IDR (TBP Human)

Expression

Mammalian

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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61426-hU6-shmFmr1-EF1a-mCherry

Plasmid

#157859

Purpose

Expresses a shRNA targeting mouse Fmr1 gene

Depositor

Xinyu Zhao

Article

Shen et al Nat Neurosci. 2019 Mar;22(3):386-400. do

Insert

Fmr1 (Fmr1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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retro-gfp-puro vector

Plasmid

#58249

Purpose

Retroviral expression vector encoding bicistronic EGFP and Puromycin cassette

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

EGFP

Use

Retroviral

Expression

Mammalian

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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GLDC-RES

Plasmid

#72882

Purpose

overexpression

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

GLDC (GLDC Human)

Use

Lentiviral

Expression

Mammalian

Mutation

silent mutations in site corresponding to shGLDC_2

Promoter

CMV

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only
cryS96

Plasmid

#67519

Purpose

αB-crystallin minipeptide fused to ELP (S96).

Depositor

Andrew MacKay

Article

Wang et al J Control Release. 2014 Oct 10;191:4-14.

Insert

mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Synthetic, Human)

Tags

elastin-like polypeptide (ELP) S96

Expression

Bacterial

Mutation

mini-αB-crystallin contains residues 73–92 of aB-…

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pGem-sHyper:sAPX

Plasmid

#84738

Purpose

Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

sAPX (SAPX Mustard Weed)
sHyper (HyPer2 targeted to nucleus)

Tags

nuclear localisation signals from the coding sequ…

Expression

Plant

Promoter

pFMV/p35S

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer:sAPX

Plasmid

#84735

Purpose

Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

sAPX (SAPX Mustard Weed)
nHyper (HyPer2 targeted to nucleus)

Tags

nuclear localisation signals from the coding sequ…

Expression

Plant

Promoter

FMV/35S

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Twin-Strep-TEV-DGKζ

Plasmid

#202531

Purpose

Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Insert

DGKZ (DGKZ Human)

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Promoter

CAG and chicken β-actin promoter

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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EIF2B3

Plasmid

#172072

Purpose

In vitro transcription for rescue experiments

Depositor

Cheol-Hee Kim

Article

Lee et al Hum Mol Genet. 2021 Apr 27;30(5):331-342

Insert

EIF2B3 (EIF2B3 Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

July 18, 2021

Availability

Academic Institutions and Nonprofits only

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