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pMW37: pRS426-R2Bm RNA

Plasmid

#209291

Purpose

Contains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequences

Depositor

Feng Zhang

Article

Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

Insert

R2Bm 5'UTR and 3'UTR

Expression

Bacterial and Yeast

Promoter

GAL1 GAPDH

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-His tagged nuclear Pif1

Plasmid

#65047

Purpose

expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

Depositor

Jean-Baptiste Boulé, Virgina Zakian

Article

Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

Insert

Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)

Tags

histag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

Plasmid

#35152

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5, pTrc

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pBAF

Plasmid

#104152

Purpose

Plasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.

Depositor

Robert Craigie

Article

Lee et al Proc Natl Acad Sci U S A. 1998 Feb 17;95

Insert

Barrier-to-autointegration factor (BAF) (BANF1 Human)

Tags

N-terminal His tag with thrombin cleavage site

Expression

Bacterial

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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4xHRE-MinTK-CRE

Plasmid

#141144

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

4xHRE-MinTK-CRE

Use

Lentiviral

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-FLAG-TurboID

Plasmid

#223713

Purpose

Expresses Flag-tagged TurboID

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

TurboID

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNMT

Plasmid

#42578

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

N-myristoyl tranferase (NMT1 Budding Yeast)

Expression

Bacterial

Mutation

two silent mutations T690C and T942C

Promoter

T7

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-hNox4

Plasmid

#69352

Purpose

expresses human Nox4 in mammalian cells

Depositor

Karl-Heinz Krause

Article

Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

Insert

hNOX4 (NOX4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt5

Plasmid

#184506

Purpose

Mammalian expression of Synaptotagmin 5 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 5 (Syt5 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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plRL117

Plasmid

#163635

Purpose

Optimized M. smegmatis CRISPRi plasmid; L5 integrating.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Tet-On

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEIGW roGFP2-Orp1

Plasmid

#64993

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-hIFITM3

Plasmid

#58397

Purpose

Expressed human IFITM3 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (IFITM3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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UAS:Zebrabow-B

Plasmid

#118216

Purpose

For broad gal4 inducible expression of Brainbow in zebrafish

Depositor

Albert Pan, Alex Schier

Article

Pan et al Development. 2013 Jul;140(13):2835-46. d

Insert

Brainbow 1.0 L

Use

Zebrafish expression

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57-RFP-P2A-MCS

Plasmid

#78933

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-hAgo2

Plasmid

#21981

Depositor

Phil Sharp

Article

Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

Insert

Argonaute 2 (AGO2 Human)

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pGWB402SN

Plasmid

#224551

Purpose

Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

Depositor

Paul Schulze-Lefert

Article

Lawson et al bioRxiv 2024.07.14.603419

Type

Empty backbone

Tags

HA-Twin-Strep

Expression

Plant

Promoter

2 X 35S

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-hIFITM1

Plasmid

#58399

Purpose

Expressed human IFITM1 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM1 (IFITM1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pLPCX roGFP2-Orp1

Plasmid

#64991

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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dCas12j2 KRAB

Plasmid

#188508

Purpose

Expresses FLAG tagged dCas12j2 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j2

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D394A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCS6-SMASh-YFP

Plasmid

#68852

Purpose

Expresses SMASh tag fused to YFP at N terminus in mammalian cells

Depositor

Michael Lin

Article

Chung et al Nat Chem Biol. 2015 Jul 27. doi: 10.1038

Insert

SMASh-YFP

Tags

SMASh tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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