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pT2MP.EF-QNA

Plasmid

#129306

Purpose

Negative control for ATP measurement, Tol2 transposon

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-NA

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pILGFP4L

Plasmid

#104507

Purpose

Used to evaluate the expression output of Saccharomyces mikatae GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SmGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Nov. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pGC4780

Plasmid

#113408

Purpose

Translation reporter with inducible translation coupling (high native GFP expression)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Insert

158_33121123_1

Use

Synthetic Biology

Tags

fused to codon deptimized sfGFP with Flexible lin…

Expression

Bacterial

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pTH646-2µ-RLuc

Plasmid

#40600

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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SNAP-EF(N)-30nmSAH-GFP(1-10)

Plasmid

#61662

Purpose

30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-30nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pILGFP4H

Plasmid

#104503

Purpose

Used to evaluate the expression output of Saccharomyces arboricola GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SaGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4J

Plasmid

#104505

Purpose

Used to evaluate the expression output of Saccharomyces mikatae GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SmGAL1 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4N

Plasmid

#104509

Purpose

Used to evaluate the expression output of Saccharomyces bayanus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SbGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP4R

Plasmid

#104513

Purpose

Used to evaluate the expression output of Saccharomyces uvarum GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SuGAL2 promoter-yEGFP

Expression

Yeast

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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POT4-TDH3p-ycrtI-ADH1t

Plasmid

#65355

Purpose

TU for combinatorial construction of ?-carotene pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

TDH3p-ycrtI-ADH1t

Use

Unspecified

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pDS255

Plasmid

#34970

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

T406A, T407A

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCSJ125

Plasmid

#104344

Purpose

Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

MDH2

Tags

3xFLAG

Expression

Yeast

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean3-MannII-N-10

Plasmid

#55429

Purpose

Localization: Mannosidase/Golgi, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

MannII

Tags

mCerulean3

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pTYF-2xGfaABC1D-tTA

Plasmid

#19981

Depositor

Sergey Kasparov

Article

Liu et al J Gene Med. 2008 May . 10(5):583-92.

Insert

2xGfaABC1D-tTA

Expression

Mammalian

Available Since

Aug. 6, 2009

Availability

Academic Institutions and Nonprofits only

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pRS416 Hsh155 WT

Plasmid

#87219

Purpose

Plasmid used to maintain viability after deletion of genomic copy of HSH155.

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pG1-NMGR526

Plasmid

#1122

Depositor

Susan Lindquist

Article

Li et al Science 2000 Jan 28;287(5453):661-4.

Insert

sup35 NM domain fused with GR (SUP35, Nr3c1 Budding Yeast, Rat)

Tags

GR526

Expression

Yeast

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only
pAG416GPD-SEC7-Cerulean

Plasmid

#18851

Depositor

Susan Lindquist

Article

Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

Insert

SEC7 (SEC7 Budding Yeast)

Tags

Cerulean

Expression

Yeast

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pET-Cdc12-Cdc11

Plasmid

#131162

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

6his-Cdc12 + Cdc11

Use

Bicistronic

Tags

Cdc11: S-Tag and Cdc12: 6his

Expression

Bacterial

Promoter

T7

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAc-Cdc3-Cdc10

Plasmid

#131160

Purpose

Expression of Septin rods

Depositor

Nils Johnsson

Article

Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

Insert

Cdc3 + Cdc10

Use

Bicistronic

Tags

Cdc10: S Tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFA1

Plasmid

#46789

Purpose

To express Fun30 in trans in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Promoter

PGAL1

Available Since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only