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MigR1 Fli-1 GFP

Plasmid

#24668

Depositor

Yaacov Ben-David

Article

Cui et al Blood. 2007 Oct 1. 110(7):2631-40.

Insert

Fli-1 (Fli1 Mouse)

Use

Retroviral

Tags

GFP and IRES

Available Since

May 6, 2010

Availability

Academic Institutions and Nonprofits only
FLAG-β-arrestin2 RRK/Q

Plasmid

#38261

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag

Expression

Mammalian

Mutation

Arg233, Arg237, and Lys251 converted to Glutamines

Promoter

CMV

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pMDC32B-OsMIR390-B/c

Plasmid

#61467

Purpose

Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

Depositor

James Carrington

Article

Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

Insert

OsMIR390-B/c

Expression

Plant

Mutation

O. sativa MIR390 precursor sequence including a c…

Promoter

2x35S

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11

Plasmid

#192147

Purpose

T cell CRISPR lentiviral vector

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Type

Empty backbone

Expression

Mammalian

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2alpha WT

Plasmid

#128812

Purpose

To make virus expressing Doc2alpha WT

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta WT

Plasmid

#128814

Purpose

To make virus expressing Doc2beta WT

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX Txk-SH2

Plasmid

#46526

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Txk (Txk Mouse)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 138-258

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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Ai39

Plasmid

#34884

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2012 Mar 25;15(5):793-802.

Insert

CAG-Floxed eNpHRv3.0-EYFP

Use

Mouse Targeting

Tags

EYFP

Promoter

CAG

Available Since

Feb. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_1

Plasmid

#73533

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_2

Plasmid

#73534

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pET-51b(+)-Caprola_14

Plasmid

#194675

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_14

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX Sh3bp2-SH2

Plasmid

#46484

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Sh3bp2 (SH3BP2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 450-561

Promoter

Tac

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PBD HK-AA (Nigg AH13)

Plasmid

#41161

Depositor

Erich Nigg

Article

Hanisch et al Mol Biol Cell. 2006 Jan;17(1):448-59. Ep

Insert

PBD HK-AA (PLK1 Human)

Tags

3xMyc and PreScission Site

Expression

Mammalian

Mutation

Contains Plk1-PBD only (aa 326–603); HK-AA (H538A…

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only
LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro

Plasmid

#61441

Purpose

Dox-inducible MyoD with mRNA tag for smFISH

Depositor

Charles Gersbach

Article

Gibson et al Integr Biol (Camb). 2015 Jun 8;7(6):663-

Insert

MyoD (Myod1 Mouse)

Use

Lentiviral

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 HA-Arp8

Plasmid

#19796

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

Arp8 (ACTR8 Human)

Tags

2XHA

Expression

Mammalian

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 MYC dLZ

Plasmid

#176048

Purpose

Expresses Myc Mutant (delta leucine zipper) in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Expression

Mammalian

Mutation

deleted amino acids 414-433

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_OTmut

Plasmid

#185385

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in mammalian cells

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin control sensor GRAB_OTmut

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Enhancer.1

Plasmid

#78536

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Supt6h-SH2

Plasmid

#46518

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Supt6h (SUPT6H Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1317-1441

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA

Plasmid

#191690

Purpose

For knocking p2a-EGFP into OTX2 locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

OTX2 homology arms and p2a-EGFP

Expression

Mammalian

Mutation

WT

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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