We narrowed to 23,367 results for: Nov;
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Plasmid#162736PurposeMammalian expressionDepositorInsertSpacer sequence for GAPDH
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-soCoChR-mCardinal
Plasmid#107713PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. hSynapsin promoterDepositorInsertsoCoChR-mCardinal
UseAAVTagsKA2 and mCardinalExpressionMammalianMutationPromoterhSynAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1(delta ZF)
Plasmid#90110PurposeMammalian expression of Flag-MCPIP1(delta ZF)DepositorInsertMCPIP1 (ZC3H12A Human)
UseTagsFlagExpressionMammalianMutationK317G, S318GPromoterCMVAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-6
Plasmid#172771PurposeBacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-6
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-TetR
Plasmid#103797PurposeBLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationTetR: A4G (M2V)PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET SUMO NL-FKBP-Cas9
Plasmid#162730PurposeBacterial expression, protein expressionDepositorInsertFKBP F36V
UseTags6xHis and SUMOExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1(150-599)
Plasmid#90108PurposeMammalian expression of Flag-MCPIP1(150-599)DepositorInsertMCPIP1 (ZC3H12A Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sumo3 PolQM1 deltains1
Plasmid#78461PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; delta 2149-2170; Q2513R (please see…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1(81-599)
Plasmid#90107PurposeMammalian expression of Flag-MCPIP1(81-599)DepositorInsertMCPIP1 (ZC3H12A Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-27
Plasmid#172752PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-27
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-Sox1
Plasmid#50778Purposeretroviral expression of mouse Sox1DepositorInsertSox1 (Sox1 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pPHR-mCherry-NLS-VP16
Plasmid#103821PurposeSoluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-mCherry-VP16 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationVP16: G126T (silent, G42G)PromoterCMVAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-24
Plasmid#172750PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-24
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-9
Plasmid#172742PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-9
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-EYFP-hPMLIII
Plasmid#103808PurposeBLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHR-iRFP713-NLS-VP16
Plasmid#103823PurposeSoluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illuminationDepositorInsertPHR-iRFP713-VP16 (CRY2 Mustard Weed)
UseTagsExpressionMammalianMutationVP16: G126T (silent, G42G)PromoterCMVAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-8
Plasmid#172761PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-8
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-5
Plasmid#172760PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-5
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSN-A112C-GpA
Plasmid#191238PurposeExpresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.DepositorInsertnuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
UseTagsnuclease A from S. aureus fused to the TM domain …ExpressionBacterialMutationChanged Alanine 112 to CysteinePromoterT7 promoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1alpha-soCoChR-GFP
Plasmid#107709PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoterDepositorInsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1(1-453)
Plasmid#90106PurposeMammalian expression of Flag-MCPIP1(1-453)DepositorInsertMCPIP1 (ZC3H12A Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCOCK CM-R03
Plasmid#46567PurposeMammalian expression plasmid with EGFP expressed from synthetic promoter (random mutagenesis of CMVwt 3' end).DepositorInsertCMV mutant promoter
UseSynthetic BiologyTagsExpressionMammalianMutationMutant version of CMV promoter (refer to Table 1 …PromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_J
Plasmid#146709PurposeInsect Expression of DmNot1DepositorInsertDmNot1 (Not1 Fly)
UseTagsExpressionInsectMutationThree non silent mutations N605S, K759M, G1999S a…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-hPMLIII
Plasmid#103825PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXylan
Plasmid#36301DepositorInsertpXylan
UseSynthetic BiologyTagsExpressionMutationPromoterTwo promoters from the E coli genome drive two di…Available sinceNov. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-2
Plasmid#172739PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCOCK CMVwt
Plasmid#46559PurposeMammalian expression plasmid with EGFP expressed from CMV wild-type promoter.DepositorInsertCMV wt promoter
UseSynthetic BiologyTagsExpressionMammalianMutationOur version has a G at base 724 (reference has C)PromoterCMVAvailable sinceSept. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1(150-453)
Plasmid#90109PurposeMammalian expression of Flag-MCPIP1(150-453)DepositorInsertMCPIP1 (ZC3H12A Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL421_MLL2_promoter
Plasmid#118696PurposeExpress MLL2/KMT2B promoter for luciferase assayDepositorInsertMLL_promoter
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only