We narrowed to 19,051 results for: Tec
-
-
pL-CRISPR.SFFV.GFP
Plasmid#57827PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-eGFP
UseCRISPR and LentiviralTagsFLAGAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)
Plasmid#199604PurposeExpression of cell permeable spCas9 in E. Coli.DepositorInsertCas9-T6N
UseCRISPRExpressionBacterialAvailable SinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pES002 MBP-GSK3β_S9A-HA-His
Plasmid#196184PurposeExpresses MBP-GSK3β_S9A-HA-His (human Glycogen Synthase Kinase 3β S9A mutant as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorInsertGSK3B (GSK3B Human)
TagsHA, His6, and MBPExpressionBacterialMutationchanged Serine 9 to AlaninePromoterTacAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-VPR_P2A_mCherry
Plasmid#154193PurposeLentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry markerDepositorInsertsp-dCas9-VPR_P2A_mCherry
UseLentiviralExpressionMammalianMutationD10A, H840APromoterEF1asAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET19-M(BRCT)TdTwt
Plasmid#207459PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domainDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 TadCBEd-V106W
Plasmid#193839PurposeExpress TadCBEd-V106W (with SpCas9) in mammalian cellsDepositorInsertTadA-CDd V106W-SpCas9 D10A-2xUGI
ExpressionMammalianAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC39A10
Plasmid#132275PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC39A10 (SLC39A10 Human)
ExpressionMammalianAvailable SinceNov. 14, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEf1a-superDn29-P2A-GFP
Plasmid#247152PurposeMammalian expression of a human codon optimized engineered superDn29 recombinase (highest efficiency variant)DepositorInsertsuperDn29-P2A-GFP
Tags6xHisExpressionMammalianMutationM6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…PromoterEf1aAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
B*35:03:01
Plasmid#203255PurposeOverexpress HLA alleleDepositorInsertB*35:03:01 (HLA-B Human)
ExpressionMammalianAvailable SinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-tdTomato-P2A-BlasR (LRT2B)
Plasmid#110854PurposeLentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markersDepositorInsertsgRNA scaffold with spacer
UseLentiviralMutationWTPromoterU6Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE
Plasmid#138148PurposeExpresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInsertCasRx
UseLentiviralTagsNESAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dCas13-M3nls
Plasmid#155366PurposeTargeted m6A RNA methylation in mammalian cellsDepositorInsertdCas13-M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
UseCRISPRExpressionMammalianMutationPspCas13b Δ984-1090 H133AAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dual-sgRNA_hU6-mU6
Plasmid#154194PurposeLentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDisCoTune
Plasmid#173713PurposePromotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.DepositorInsertsExpressionBacterialAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-C-ABE8e
Plasmid#185667PurposeExpresses eNme2-C-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-C
UseCRISPRExpressionMammalianMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…Available SinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.2.t1
Plasmid#73546PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.2.t1
pAzFRS.2.t1
ExpressionBacterialAvailable SinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE
Plasmid#138147PurposeExpresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInsertCasRx
UseLentiviralTagsNLSAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL1521 (pSPIN, pSC101* backbone)
Plasmid#160729PurposeSingle-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEf1a-goldDn29-P2A-GFP
Plasmid#247153PurposeMammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant)DepositorInsertgoldDn29-P2A-GFP
Tags6xHisExpressionMammalianMutationM6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503NPromoterEf1aAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only
