We narrowed to 13,724 results for: bli

1. pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa

   Plasmid

   #221352

   Purpose

   Mammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa; to be used with Anti-EE [IPI-3D5EE_48.K] heavy chain (Plasmid 221351) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pTipi2.1_Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC

   Plasmid

   #221351

   Purpose

   Mammalian expression of Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC; to be used with Anti-EE [IPI-3D5EE_48.K] light chain (Plasmid 221352) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-EE [IPI-3D5EE_48.K] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. AICSDP-80:UBTF-HaloTag

   Plasmid

   #133963

   Purpose

   Homology arms and HaloTag-linker sequence for N-terminus tagging of human UBTF

   Depositor

   Allen Institute for Cell Science

   Insert

   UBTF Homology Arms with HaloTag-linker (UBTF Human)
   

   Use

   CRISPR

   Tags

   HaloTag-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRN3P_EGFP_m6A_Tracer

   Plasmid

   #139403

   Purpose

   in vitro transcription for mRNA

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Borsos et al Nature. 2019 May;569(7758):729-733. doi:

   Insert

   m6A-Tracer
   

   Use

   In vitro transcription

   Tags

   EGFP

   Promoter

   T3

   Available Since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Membrane EGFP

   Plasmid

   #198056

   Purpose

   Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence

   Depositor

   Amro Hamdoun

   Insert

   LCK membrane EGFP (LCK Synthetic, Human, A. victoria)
   

   Use

   Sea urchin, mammal, zebrafish

   Tags

   EGFP

   Mutation

   Tandem repeat of N-terminal LCK membrane targetin…

   Promoter

   sp6

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPyCAG-mKate2-3xNLS-IRES-Pac

   Plasmid

   #183618

   Purpose

   Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

   Insert

   mKate2-3xNLS
   

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti Puro HA-Ubiquitin-K48

   Plasmid

   #236290

   Purpose

   Lentiviral vector expressing HA-tagged ubiquitin K48 mutant

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Ubiquitin K48 (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   Single ubiquitin coding sequence containing K48 m…

   Promoter

   CMV

   Available Since

   April 10, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLenti Puro HA-Ubiquitin-K63

   Plasmid

   #236291

   Purpose

   Lentiviral vector expressing HA-tagged ubiquitin K63 mutant

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   Ubiquitin K63 (UBB Human)
   

   Use

   Lentiviral

   Tags

   HA

   Mutation

   Single ubiquitin coding sequence containing K63 m…

   Promoter

   CMV

   Available Since

   April 10, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pJ151-HDR

   Plasmid

   #113631

   Purpose

   Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

   Depositor

   Alex Kentsis

   Inserts

   Left homology arm
   Puromycin resistance gene
   Venus fluorescence gene
   Thymidine kinase
   Right homology arm
   

   Use

   Cre/Lox; Homology recombination selection vector

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a promoter, EF1a promoter (T2A cleaving sequen…

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AICSDP-57: SMC1A-mEGFP

    Plasmid

    #114406

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A

    Depositor

    Allen Institute for Cell Science

    Insert

    SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAAV/hSyn-SCLM

    Plasmid

    #216760

    Purpose

    AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon

    Depositor

    George Augustine

    Insert

    SuperClomeleon
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    hSyn (human synapsin I)

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-osTIR1-9xMyc-Zeo

    Plasmid

    #225696

    Purpose

    Lentiviral expression of 9xMycTag fused osTIR1and Zeocin resistance. For use with AID degron.

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    osTIR1
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    9X Myc

    Expression

    Mammalian

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQTEV-STMN1

    Plasmid

    #31326

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    stathmin 1/oncoprotein 18 (STMN1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKOR1-mcs

    Plasmid

    #181755

    Purpose

    Allele replacement for Staphylococcus aureus

    Depositor

    Suzan Rooijakkers

    Article

    Stapels et al Proc Natl Acad Sci U S A. 2014 Sep 9;111

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMTEZ-Kir2.1

    Plasmid

    #32641

    Depositor

    Joseph Gogos, Richard Axel, C. Ron Yu

    Article

    Yu et al Neuron. 2004 May 27;42(4):553-66.

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Expression

    Bacterial

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. ITCH

    Plasmid

    #36197

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ITCH (ITCH Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains HECT domain

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pCDB179-LCC

    Plasmid

    #218686

    Purpose

    Encodes codon optimized LCC enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 2xMARS-nGFP

    Plasmid

    #205235

    Purpose

    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

    Depositor

    Jeremy Baskin

    Insert

    2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
    

    Tags

    HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-sCAG-eGFP-RAB11

    Plasmid

    #203799

    Purpose

    AAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    RAB11 (RAB11A Human)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-PGK-CXCR4 (neo)

    Plasmid

    #192077

    Purpose

    Lentivirus for expression of CXCR4

    Depositor

    Roland Friedel

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Lentiviral

    Promoter

    hPGK

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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