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mNG2(1-10)_tdTomato

Plasmid

#184169

Purpose

Lentiviral vector for expression and selection of the mNG2(1-10) split fluorescent protein.

Depositor

Ophir Shalem

Article

Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

Inserts

mNG2(1-10)
tdTomato

Use

Lentiviral

Expression

Mammalian

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD4

Plasmid

#121108

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N601A) (PBRM1 Human)

Use

Lentiviral

Mutation

PBRM1 (N601A)

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD5

Plasmid

#121109

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N739A) (PBRM1 Human)

Use

Lentiviral

Mutation

PBRM1 (N739A)

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD1

Plasmid

#121105

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (Y127A) (PBRM1 Human)

Use

Lentiviral

Mutation

PBRM1 (Y127A)

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD3

Plasmid

#121107

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N463A) (PBRM1 Human)

Use

Lentiviral

Mutation

PBRM1 (N463A)

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw PBRM1 mBD2

Plasmid

#121106

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Porter et al J Biol Chem. 2017 Jan 4. pii: jbc.M116.7

Insert

PBRM1 (N263A) (PBRM1 Human)

Use

Lentiviral

Mutation

PBRM1 (N263A)

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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ARTi

Plasmid

#214064

Purpose

Fluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

TurboRFP

Expression

Mammalian

Mutation

Linker sequence present between RFP and GFP

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Fab_8D3_2_H-H6

Plasmid

#176075

Purpose

Express His6 tagged heavy chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

His6 tagged heavy chain of Fab_8D3_2

Tags

His tag

Expression

Mammalian

Promoter

CAG promoter

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Fab_8D3_2_L

Plasmid

#176076

Purpose

Express light chain of Fab_8D3_2 in mammalian cells

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

Light chain of Fab_8D3_2

Expression

Mammalian

Promoter

CAG promoter

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-HA-hUBE1L

Plasmid

#12438

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

UBE1L (UBA7 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pMK347 (BSD-P2A-mAID)

Plasmid

#121181

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

BSD-P2A-mAID

Use

CRISPR

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-G-Flamp2-mut

Plasmid

#192783

Purpose

control probe of G-Flamp2, insensitive to cAMP

Depositor

Jun Chu

Article

Liu et al Front Pharmacol. 2022 May 12;13:902290.

Insert

G-Flamp2-mut

Tags

His tag

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAG1159 TOM20-ECFP-FIREmate

Plasmid

#196693

Purpose

Expresses TOM20-ECFP-FIREmate in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

TOM20-ECFP-FIREmate

Tags

FIREmate

Expression

Mammalian

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV4-3 HA/IkB-alpha

Plasmid

#21985

Depositor

Warner Greene

Article

Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

Insert

I-kappa B alpha (NFKBIA Human)

Tags

3x HA

Expression

Mammalian

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pAG1160 mCherry-FIREtag

Plasmid

#205164

Purpose

Expresses mCherry -FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK.NeuroD2.WPRE

Plasmid

#122054

Purpose

Lentiviral expression of NeuroD2

Depositor

Stephen Tapscott

Article

Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

Insert

NeuroD2 (Neurod2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRecT_2

Plasmid

#167546

Purpose

Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.

Depositor

Howard Hang

Article

Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

Insert

E. faecium Com15 recT under an IPTG inducible promoter, with lacI

Expression

Bacterial

Promoter

IPTG

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV3flag8SHARPIN

Plasmid

#50014

Purpose

Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

Depositor

Martin Dorf

Article

Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

Insert

SHANK-associated RH domain interactor (SHARPIN Human)

Tags

3 x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 31, 2013

Availability

Academic Institutions and Nonprofits only

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DHFR-myc

Plasmid

#20214

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

DHFR (folA E. coli)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pATT-BsaI-Pmag-NmagHigh1

Plasmid

#202713

Purpose

Contains Magnet flanked by linkers and BsaI sites insert for transposon insertion libraries.

Depositor

Mary Dunlop

Article

Tague et al ACS Synth Biol. 2023 Oct 3. doi: 10.1021

Insert

linker-pMag-RBS-nMagHigh1-linker

Use

Staging plasmid to make transposon insertion libr…

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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