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mChF-Cav1s

Plasmid

#171464

Purpose

Expresses mChF-Cav1s, a FiCE of iCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

mCherry-FKBP12-Caveolin1(61-178 aa)

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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YF-Cav1s

Plasmid

#171462

Purpose

Expresses YF-Cav1s, a FiCE of iCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

EYFP-FKBP12-Caveolin1(61-178 aa)

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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mActZ-FY

Plasmid

#171463

Purpose

Expresses mActZ-FY, a FiCE of iCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

ActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-EYFP

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS-HsProm1s1(W795R)-StayGold

Plasmid

#211334

Purpose

Vector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (W795R)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Use

AAV

Tags

StayGold

Mutation

Changed Trp-795 to Arg

Promoter

EF1a

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1(W795R)-TwinStrep-His

Plasmid

#210817

Purpose

Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (W795R)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

TwinStrep, 10xHis

Expression

Mammalian

Mutation

Changed Trp-795 to Arg

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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Src(KD/YF)-SsrA(Y7I)-mCer3

Plasmid

#221000

Purpose

this is microTag (Y7I mutation in SsrA)-Src(D388R/Y529F) with mCerulean3 at C-terminal

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA, mCerulean3 (Src Mouse)

Tags

mCerulean3

Expression

Mammalian

Mutation

D388R/Y529F in Src, Y7I in SsrA

Promoter

pCMV

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a(+)_co_L-HicDH

Plasmid

#185453

Purpose

CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

Depositor

Wolfgang Kroutil

Article

Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

Insert

L-HicDH from Lactobacillus confusus DSM 20196

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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mYF

Plasmid

#171460

Purpose

Expresses mYF, a CiCE of NiCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

EYFP(A207K)-FKBP12

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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mActZ-FmCh

Plasmid

#171465

Purpose

Expresses mActZ-FmCh, a FiCE of iCMM, in mammalian cells

Depositor

Takafumi Miyamoto

Article

Miyamoto et al Cell Rep Methods. 2021 Jul 22;1(4):10005

Insert

ActA(30-262 aa)-Zyxin(2-380 aa)-FKBP12-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-HsPcdh21-mNeonGreen

Plasmid

#210819

Purpose

Mammalian overexpression vector for C-terminally mNeonGreen tagged human Pchd21 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Protocadherin 21 (CDHR1 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-Pink1

Plasmid

#101874

Purpose

Transient expression of Pink1-YFP in mammalian cells

Depositor

Richard Youle

Article

Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

Insert

PTEN induced putative kinase 1 (PINK1 Human)

Tags

fluorescent tag EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pD21-AI-hLDLR

Plasmid

#124964

Purpose

Express human LDLR under human APOAI promoter

Depositor

Kazuhiro Oka

Article

Li et al J Genet Syndr Gene Ther. 2011 Sep 28;2:1

Insert

human LDLR (LDLR Human)

Use

Adenoviral

Promoter

human APOAI

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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GST-TEV-FUS

Plasmid

#29629

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Tags

GST and TEV

Expression

Bacterial

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only

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p7SL-neo-TET

Plasmid

#51285

Purpose

expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

7SL RNA (RN7SL2 Human)

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Promoter

7SL upstream pollII enhancer

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSEVA251_ Ptrc.x.tetO2::D-HicDH

Plasmid

#185456

Purpose

CDS of D-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 under the control of the synthetic promoter Ptrc.x.tetO2 and the RBS BBa_B0030 in pSEVA251 plasmid.

Depositor

Wolfgang Kroutil

Article

Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

Insert

D-HicDH from Lactobacillus paracasei DSM 20008

Expression

Bacterial

Promoter

Ptrc.x.tetO2

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#159281

Purpose

A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

AsCpf1-HA-2A-GFP

Use

Multicistronic vector with both caggs promoter-dr…

Tags

3X HA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-neo-TET

Plasmid

#51286

Purpose

expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

U6 (RNU6-1 Human)

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Promoter

U6 upstream enhancer sequence

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pHS1928

Plasmid

#205967

Purpose

JAGBLH010000220 Cas8-HNH operon (E. coli codon optimized) with His-tagged Cas8 in pET45b(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH all proteins, with His-tagged Cas8

Tags

14xHis on Cas8

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1861

Plasmid

#205974

Purpose

FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1517

Plasmid

#205980

Purpose

FLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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