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J23100-mRFP1-331Bb

Plasmid

#78271

Purpose

mRFP1 behind constitutive promoter J23100 in pSEVA331Bb backbone

Depositor

Tom Ellis

Publication

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23100-mRFP1

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV6M-Mst2

Plasmid

#84296

Purpose

Expresses Myc-tagged Mst2

Depositor

Jonathan Chernoff

Publication

Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

Insert

Mst2 (STK3 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Oct. 19, 2016

Availability

Academic Institutions and Nonprofits only
pRSF-DUET Cas1 Cas2

Plasmid

#80088

Purpose

Encodes Cas1 and Cas2

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 E. coli (K12))
Cas2

Expression

Bacterial

Promoter

T7

Available since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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mCitrine-USP19

Plasmid

#78593

Purpose

Express mCitrine-USP19 in mammalian cells

Depositor

Yihong Ye

Publication

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 (USP19 Human)

Tags

mCitrine

Expression

Mammalian

Promoter

CMV

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
GPR162-Tango

Plasmid

#66335

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

GPR162 (GPR162 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pDGE1109

Plasmid

#202773

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Publication

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_ttriple
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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KHBD00621

Plasmid

#39693

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG31392 (CG42726 Fly)

Use

Gateway Cloning

Promoter

none

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-CR-CT

Plasmid

#18069

Depositor

Marius Sudol

Publication

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
GST-GNU-N

Plasmid

#112972

Purpose

Expresses fusion of GST and N terminal amino acids of GNU

Depositor

Terry Orr-Weaver

Publication

Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

Insert

gnu (gnu Fly)

Expression

Bacterial

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 gnu

Plasmid

#112999

Purpose

For protein expression and purification of full-length Drosophila gnu

Depositor

Terry Orr-Weaver

Publication

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

full length gnu (gnu Fly)

Expression

Bacterial

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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gnu-GFP WT

Plasmid

#113005

Purpose

P element vector for transposon insertion of gnu-GFP WT into Drosophila genome

Depositor

Terry Orr-Weaver

Publication

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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gnu cDNA LD12084

Plasmid

#112973

Purpose

Cloning vector carrying the gnu cDNA

Depositor

Terry Orr-Weaver

Publication

Lee et al Genes Dev. 2003 Dec 1;17(23):2979-91. do

Insert

gnu (gnu Fly)

Expression

Bacterial

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1 gnu 9A

Plasmid

#113000

Purpose

For protein expression and purification of full-length Drosophila gnu 9A

Depositor

Terry Orr-Weaver

Publication

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Expression

Bacterial

Mutation

9 CycB/CDK1 sites mutated to A

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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gnu-GFP 9A

Plasmid

#113006

Purpose

P element vector for transposon insertion of gnu-GFP 9A into Drosophila genome

Depositor

Terry Orr-Weaver

Publication

Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

Insert

full length gnu (gnu Fly)

Use

P insertion vector

Tags

GFP

Mutation

9 CycB/CDK1 sites mutated to A

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-BTLA (Y226F, Y243F, Y257F, Y282F)-mGFP

Plasmid

#180792

Purpose

Expression of human BTLA (Y226F, Y243F, Y257F, Y282F) fused to mEGFP

Depositor

Enfu Hui

Publication

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLA (BTLA Human)

Use

Lentiviral

Tags

mEGFP

Mutation

Y226F, Y243F, Y257F, Y282F

Promoter

SFFV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE18

Plasmid

#90360

Purpose

TGFb - SMAD2/SMAD3 gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

TGFb

Available since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAC150-KEIMA-RAMP4

Plasmid

#201929

Purpose

PiggyBac Vector expressing KEIMA-RAMP4

Depositor

Wade Harper

Publication

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

RAMP4 (SERP1 Human)

Expression

Mammalian

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AW01_pU.CAG.eSpCas9(1.1).rBGpA

Plasmid

#199255

Purpose

Expression construct encoding the high specificity eSpCas9(1.1) nuclease

Depositor

Manuel Goncalves

Publication

Chen et al Nucleic Acids Res. 2016 Jun 8. pii: gkw5

Insert

High specificity eSpCas9(1.1) nuclease

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

K848A K1003A R1060A

Promoter

CAG promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE25

Plasmid

#90367

Purpose

Notch - NIDC/CBF1 gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

Notch

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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hHS1-M7A,H102A

Plasmid

#159171

Purpose

Human codon-optimized cytosolic labile heme reporter

Depositor

Amit Reddi

Publication

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

hHS1-M7A,H102A

Expression

Mammalian

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

CMV

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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