Addgene

We narrowed to 1,130 results for: SAM-2

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MTK1_032

Plasmid

#123694

Purpose

Encodes ConL3 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L3-P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_033

Plasmid

#123695

Purpose

Encodes ConL4 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L4-P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_034

Plasmid

#123696

Purpose

Encodes ConL5 and picornavius peptide 2A (P2A) element as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-P2A

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_012

Plasmid

#123675

Purpose

Encodes ConL4 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L4-2xHS4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_013

Plasmid

#123676

Purpose

Encodes ConL5 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-2xHS4

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pYPS1-Venus

Plasmid

#133118

Purpose

Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pYPS1 driving a Venus.

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

pYPS1-YFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pCMK2-Venus

Plasmid

#133119

Purpose

Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pCMK2 driving a Venus.

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

pCMK2-YFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGYP7-Venus

Plasmid

#133120

Purpose

Plasmid contains a reporter gene for Crz1. The 1000bp promoter of the gene pGYP7 driving a Venus.

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

pGYP7-YFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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MTK234_026

Plasmid

#123906

Purpose

Encodes the spCas9 human non-targeting gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

human non targeting sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Olig001

Plasmid

#170716

Purpose

Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

Depositor

Thomas McCown

Article

Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

Insert

Olig001 capsid gene / rep 2

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A4

Plasmid

#216823

Purpose

Expresses acyclovir-controllable IRE variant #A4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A4

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8

Plasmid

#216824

Purpose

Expresses acyclovir-controllable IRE variant #A8 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-3

Plasmid

#216825

Purpose

Expresses acyclovir-controllable IRE variant #A8-3 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8-3

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-puro-U6+27-F30-Tornado-Acyclovir-IRE#A8-4

Plasmid

#216826

Purpose

Expresses acyclovir-controllable IRE variant #A8-4 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Acyclovir-IRE#A8-4

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV R-CEPIA1er

Plasmid

#58216

Purpose

Red fluorescent indicator for calcium signaling in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

R-CEPIA1er

Use

Tags

myc

Expression

Mammalian

Mutation

R-GECO1 E300D M305L D329N F332I E336D N346K F361W…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV G-CEPIA1er

Plasmid

#58215

Purpose

Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

G-CEPIA1er

Use

Tags

myc

Expression

Mammalian

Mutation

G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

Promoter

CMV

Available since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pHrD-IRES-Fluc

Plasmid

#194250

Purpose

Human RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!

Depositor

Samson Jacob

Article

McCool et al RNA. 2022 Nov 2. pii: rna.079149.122. do

Insert

Firefly luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

human rDNA promoter and beginning of 5' ETS …

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pU6T7G

Plasmid

#71463

Purpose

Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (1 G at initiation)

Depositor

Willie Mark

Article

Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

human U6/phage T7

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Squash-FTL-IRE

Plasmid

#216828

Purpose

Expresses a circular RNA containing a Squash aptamer and an IRE (FTL derived) in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Squash-FTL-IRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-puro-U6+27-F30-Tornado-Squash-TfR-IRE

Plasmid

#216829

Purpose

Expresses a circular RNA containing a Squash aptamer and an IRE (TfR derived) in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Squash-TfR-IRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6+27

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge