Addgene

We narrowed to 21,833 results for: Sis

Showing: 761 - 780 of 21833 results

Results per page:

Sort results by:

pBHDuet173

Plasmid

#118398

Purpose

analysis of cis-splicing by mini inteins

Depositor

Hideo Iwai

Article

Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

Insert

MvuTFIIB mini intein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-At-TRL

Plasmid

#32242

Depositor

Jay Hesselberth, Stan Fields

Article

Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

Insert

A. thaliana tRNA ligase (RNL Mustard Weed)

Use

Tags

6xHistidine

Expression

Bacterial

Mutation

Promoter

T7 RNA Polmyerase

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSDONR P1-P4

Plasmid

#71744

Purpose

ampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloning

Depositor

Roger Innes

Article

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Use

Gateway entry vector for use in multisite gateway…

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBUN276

Plasmid

#60673

Purpose

overexpression of M. tuberculosis ddl gene in Mycobacterium

Depositor

Raul Barletta

Article

Feng et al Antimicrob Agents Chemother. 2003 Jan;47

Insert

ddl

Use

E.coli-mycobacterium shuttle vector

Tags

6 codons of M. bovis BCG hsp60

Expression

Mutation

Promoter

BCG Hsp60

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pBHDuet50K

Plasmid

#118399

Purpose

analysis of cis-splicing by mini inteins

Depositor

Hideo Iwai

Article

Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

Insert

MvuTFIIB mini intein

Use

Tags

Expression

Bacterial

Mutation

incorporation of oligonucleotide during cloning

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2TAL3_DD_NvFP-7R_Ex2-LEFT

Plasmid

#90036

Purpose

TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

Depositor

Matthew Gibson

Article

Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

Insert

7R_Ex2-LEFT

Use

TALEN

Tags

3x Flag and Fok1-DD domain

Expression

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2TAL3_RR_NvFP-7R_Ex2-RIGHT

Plasmid

#90037

Purpose

TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

Depositor

Matthew Gibson

Article

Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

Insert

7R_Ex2-RIGHT

Use

TALEN

Tags

Fok1-RR domain and HA

Expression

Mutation

Promoter

CMV

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSXM2-pLacI-mtrCAB

Plasmid

#174618

Purpose

Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

Depositor

I-Son Ng

Article

I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Insert

mtrCAB

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLacI

Available since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMDC32-SEIPIN1

Plasmid

#96973

Purpose

Express Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSXM2-pLacI-mtrC

Plasmid

#174615

Purpose

Bacterial expression of mtrC from Shewanella oneidensis MR-1.

Depositor

I-Son Ng

Article

I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Insert

MtrC

Use

Synthetic Biology; Shuttle vector in e. coli and …

Tags

Expression

Bacterial

Mutation

Promoter

pLacI

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AL12R-EF-Tu

Plasmid

#161013

Purpose

PP7-tagged reporter driven by the Arabidopsis EF-Tu promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAtLCYbSK

Plasmid

#53588

Purpose

Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

Insert

lcyB (LYC Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMDC32-SEIPIN3

Plasmid

#96975

Purpose

Express Arabidopsis SEIPIN3 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN3 (AT2G34380 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pGG-PIP72

Plasmid

#196810

Purpose

Contains PMR4 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

POWDERY MILDEW RESISTANT 4

Use

Greengate (gg) entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-C1-FKBPflex-OSBP-siRNAr

Plasmid

#220066

Purpose

siRNA resistant ORP5 attcahed with anFKBP and a mCherry fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 22;7:2

Insert

OSBPL5 siRNA resistant (OSBPL5 Human)

Use

Tags

Expression

Mammalian

Mutation

siRNA resistant

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0120

Plasmid

#172791

Purpose

Level 0 module containing CDS2ns_mHSP21

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature HSP21 (44-227), Arabidopsis thaliana

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLBC0130

Plasmid

#172792

Purpose

Level 0 module containing CDS2ns_mPTAC5

Depositor

Benjamin Field

Article

Velay et al Plant Methods. 2022 May 26;18(1):69. doi

Insert

CDS mature PTAC5 (41-387), Arabidopsis thaliana

Use

Synthetic Biology

Tags

Expression

Mutation

no

Promoter

Available since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
(+)-Limonene Synthase

Plasmid

#112230

Purpose

(+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.

Depositor

Daniel Oprian

Article

Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17

Insert

(+)-Limonene Synthase

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

deleted amino acids 1-52

Promoter

Available since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH47742:: pRPS5a-PE2max-NC::tHSP

Plasmid

#227694

Purpose

Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

PE2max-NC being driven by pRPS5a and terminated by tHSP

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

pRPS5a

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
35S::GeNL/pCAMBIA1301

Plasmid

#190070

Purpose

Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana

Depositor

Takeharu Nagai

Article

Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

Insert

Green-enhanced Nano-lantern

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

April 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge