We narrowed to 17,085 results for: Por

1. prk5-TMEM192-3xHA PM mutant

   Plasmid

   #149506

   Purpose

   Transient expression of human TMEM192. Dileucine motifs LL23-24 and LL28-29 have been mutated to AA.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   Transient expression

   Tags

   3XHA

   Expression

   Mammalian

   Mutation

   p.LL23-24AA, p.LL28-29AA

   Promoter

   CMV

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST47-CYFIP2.GFP

   Plasmid

   #109140

   Purpose

   GFP-tagged CYFIP2 expression construct

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP2 (CYFIP2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

   Plasmid

   #194688

   Purpose

   hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_04-mEGFP
   

   Use

   AAV

   Tags

   mEGFP

   Expression

   Mutation

   Promoter

   hSyn1

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-uTEV1-MAVS-mTA4

   Plasmid

   #171002

   Purpose

   HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   MAVS(tmd)[Y536delinsFIVLI]

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB80-iLID-mCherry-RAB11

   Plasmid

   #174623

   Purpose

   Optogenetic coupling to recycling endosomes (RAB11) via iLID

   Depositor

   Lukas Kapitein

   Article

   Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   iLID-mCherry-RAB11 (RAB11A Synthetic, Human)
   

   Use

   Tags

   iLID-mCherry

   Expression

   Mammalian

   Mutation

   mCherry: Met1Del; RAB11: Met1Del

   Promoter

   Chicken beta-actin

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Hy_pMT Flag-Hel25E K91N

   Plasmid

   #110121

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   K91N mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hy_pMT Flag-Hel25E D193E

   Plasmid

   #110120

   Purpose

   Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   Hel25E (Hel25E Fly)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   D193E mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMRX-IP/Venus-mATG5

   Plasmid

   #58739

   Purpose

   Expresses Venus-mATG5 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   autophagy related genes 5 (Atg5 Mouse)
   

   Use

   Retroviral

   Tags

   Venus

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast

   Plasmid

   #166413

   Purpose

   express hPOT1 ΔOB in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   Protection Of Telomeres 1 (POT1 Human)
   

   Use

   Lentiviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   ΔOB deleted 125 a.a

   Promoter

   ef1-a

   Available Since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBa.GFP-KIF3C tail 411-796

    Plasmid

    #134616

    Purpose

    Mammalian expression of mouse KIF3C 411-796

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF3C (Kif3c Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT with deletion of the 410 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEasyG3_hph

    Plasmid

    #184919

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-PM-InPAkt

    Plasmid

    #181915

    Purpose

    Indicator of Phosphoinositides using Akt; targeted to the plasma membrane.

    Depositor

    Jin Zhang

    Article

    Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

    Insert

    pm-InPAkt
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Contains the following mutations with respect to …

    Promoter

    CMV

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid

    #100021

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfMatryoshCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP and T78H m…

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-Rab8a-QL

    Plasmid

    #86079

    Purpose

    A bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    hRab8A (RAB8A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Changed glutamine 67 to leucine

    Promoter

    tac

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. TetO-FUW-Bmi1

    Plasmid

    #61532

    Purpose

    Expresses Bmi1 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pRetroX-TRE3G_NT-GSDMD_I105N_

    Plasmid

    #201174

    Purpose

    Dox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

    Insert

    Gasdermin D N-terminal domain (Gsdmd Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    aa1-276 only, I105N

    Promoter

    TRE3G

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRE-UBC-Crry-T2A-GFP

    Plasmid

    #205452

    Purpose

    lentiviral plasmid for expression of mouse Crry

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    Crry (Cr1l Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221_SLC39A14

    Plasmid

    #132105

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

    Insert

    SLC39A14 (SLC39A14 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. GST-beta2(616-951)

    Plasmid

    #100743

    Purpose

    Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Long isoform

    Promoter

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR221_SLC7A3

    Plasmid

    #132301

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Rebsamen et al Life Sci Alliance. 2022 Sep 16;5(11):e20

    Insert

    SLC7A3 (SLC7A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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