We narrowed to 231,076 results for: Gene

1. pLCKO_Luciferase_sgRNA

   Plasmid

   #74190

   Purpose

   lentiviral vector expressing sgRNA targeting Luciferase

   Depositor

   Jason Moffat

   Article

   Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

   Insert

   Luciferase sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 Promoter

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CRY-Gal∆DD (B1013)

   Plasmid

   #92035

   Purpose

   Expresses CRY2 (full-length, with endogenous NLS) fused to Gal4 (aa1-65 of Gal4BD), downstream of mCherry-IRES

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   Fusion of plant CRY2 to Gal4 binding domain (AA1-…

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pExodus CMV.Trex2

   Plasmid

   #40210

   Purpose

   Mammalian expression of codon-optimized Trex2.

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

   Insert

   Three prime repair exonuclease 2 (Trex2 Mouse)
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmIL-6 mut NF-kB

   Plasmid

   #61293

   Purpose

   drives luciferase from mouse IL-6 promoter with mutation in NF-kB site

   Depositor

   Gail Bishop

   Article

   Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

   Insert

   IL-6 promoter (Il6 Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Mutated NF-kB binding site from GTGGGATTTTCCCA to…

   Promoter

   IL-6 promoter with mutant NF-kB binding site

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

5. HA-merlin

   Plasmid

   #32836

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

   Insert

   Merlin (NF2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

6. Hsp70-mplum

   Plasmid

   #45016

   Depositor

   Anna Planas

   Article

   de la Rosa et al Eur J Nucl Med Mol Imaging. 2013 Feb;40(

   Insert

   mPlum
   

   Expression

   Mammalian

   Promoter

   mouse Hspa1b

   Available Since

   May 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcU6_3 MS2 sgRNA

   Plasmid

   #92394

   Purpose

   Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   chick U6.3

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CIB-VP64 (B1016)

   Plasmid

   #92037

   Purpose

   Expresses CIB1 (Full-length, with endogenous NLS) fused to VP64 activation domain (4xVP16 inserts)

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CIB1-VP64 (CIB1 Mustard Weed)
   

   Tags

   Fusion of plant CIB1 with VP64 activation domain.…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9_sgRNA_0

   Plasmid

   #70763

   Purpose

   expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating

   Depositor

   Regine Kahmann

   Article

   Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10

   Inserts

   U6 promoter
   cas9
   tnos terminator
   

   Use

   Self-replicating in ustilago maydis, conferring c…

   Tags

   N-terminal NLS, C-terminal HA-tag +NLS

   Mutation

   Streptococcus pyogenes gene codon-optimized for U…

   Promoter

   U6 from Ustilago maydis and synthetic otef promot…

   Available Since

   Nov. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMCS.DT-A

    Plasmid

    #22730

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    DT-A
    

    Use

    Bac recombineering

    Mutation

    This is a gap-repair vector designed for retrievi…

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. proE-cad178-Luc-mEbox

    Plasmid

    #42082

    Depositor

    Kumiko Ui-Tei

    Article

    Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

    Insert

    E-cadherin (CDH1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    contains E-cadherin promoter region from -178 to …

    Promoter

    E-cadherin

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

    Plasmid

    #78101

    Purpose

    Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

    Depositor

    Michael Elowitz

    Article

    Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

    Insert

    H2B-mCherry-T2A-rTetR-EED (EED Synthetic, Human)
    

    Use

    Synthetic Biology

    Tags

    rTetR

    Expression

    Mammalian

    Promoter

    pEF alpha

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80063

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Promoter

    Sox10 promoter

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. 409 pSG5L HA RB (379-928)

    Plasmid

    #10734

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB (379-928) (RB1 Human)
    

    Expression

    Mammalian

    Mutation

    amino acids 379-928

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

15. p3xFLAG-CMV-Tlr4

    Plasmid

    #27148

    Depositor

    Bruce Beutler

    Article

    Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

    Insert

    toll-like receptor 4 (Tlr4 Mouse)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Native signal peptide sequence (original amino ac…

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pCAGGS_HA-VP35_EBOV

    Plasmid

    #103053

    Purpose

    Mammalian expression vector encoding HA-tagged Ebola virus VP35 gene under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al J Virol. 2016 Jul 27;90(16):7268-84. doi

    Insert

    VP35 (VP35 Zaire ebolavirus, Mayinga)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV4Neo-murine IL-17RC(FL)

    Plasmid

    #46864

    Purpose

    expresses mouse IL-17RC (all exons)

    Depositor

    Sarah Gaffen

    Article

    Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    IL-17RC (Il17rc Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. 608 pSG5L HA RB del ex4

    Plasmid

    #10730

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del exon4 (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of exon4

    Available Since

    Oct. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pY095

    Plasmid

    #84744

    Purpose

    Expresses huLbCpf1-T2A-GFP and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Inserts

    huLbCpf1
    GFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 498 pSG5L HA RB del663 (NAAIRS)

    Plasmid

    #10728

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del663 (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 664 to 669 were replaced by NAAIRS, a fl…

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only