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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 7821 - 7840 of 18384 results

  6. p-dCas9-JMJD2A-S288G-T289G-Hygro

    Plasmid
    #104418
    Purpose
    transient expression of dCas9-dJMJD2A fusion protein
    Depositor
    Stephan Beck
    Insert
    dJMJD2A
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    S288G-T289G
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104418-plasmid-map-sequence-id-204359

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  7. p-dCas9-dTET1-H1672Y-D1674A-Hygro

    Plasmid
    #104413
    Purpose
    transient expression of dCas9-dTET1 fusion protein
    Depositor
    Stephan Beck
    Insert
    dTET1
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    H1672Y-D1674A
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104413-plasmid-map-sequence-id-204141

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  11. pFW11-ffluc

    Plasmid
    #128487
    Purpose
    Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging
    Depositor
    Thomas Proft
    Article
    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2
    Insert
    firefly luciferase gene
    Use
    Allelic replacement vector
    Tags
    spy0535 downstream flanking region and spy0535 up…
    Promoter
    synthetic
    Available Since
    Feb. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    128487-plasmid-map-sequence-id-266752

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  12. pET-24a-CXCR4

    Plasmid
    #133431
    Purpose
    Human CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    CXCR4 (CXCR4 Human)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133431-plasmid-map-sequence-id-267724

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  13. KSP_K406

    Plasmid
    #129771
    Purpose
    Expresses Xenopus Kinesin-5 KSP head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays
    Depositor
    William Hancock
    Insert
    KSP (kif11.L Frog)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Truncation at 367, fused to kinesin-1 starting Al…
    Available Since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129771-plasmid-map-sequence-id-263572

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  14. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid
    #121171
    Purpose
    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    xFNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121171-plasmid-map-sequence-id-235381

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  15. pSPDEF.1.0-gDNA

    Plasmid
    #113796
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor SPDEF
    Depositor
    Kevin White
    Insert
    SPDEF (SPDEF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113796-plasmid-map-sequence-id-230613

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  16. pERF.1.0-gDNA

    Plasmid
    #112395
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ERF
    Depositor
    Kevin White
    Insert
    ERF (ERF Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112395-plasmid-map-sequence-id-230242

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  17. pTEAD1-donor

    Plasmid
    #112329
    Purpose
    CRISPR donor plasmid to tag human transcription factor TEAD1 with GFP
    Depositor
    Kevin White
    Insert
    TEAD1 homology arms flanking EGFP-IRES-Neo cassette (TEAD1 Human)
    Use
    CRISPR
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112329-plasmid-map-sequence-id-228556

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  18. pMSCV_puro_41585

    Plasmid
    #111631
    Purpose
    Complementation of Dicer_KO mESCs with mutated human DICER isoform
    Depositor
    Constance Ciaudo
    Insert
    FLAG-hsDicer (D1320A/D1709A) (DICER1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D1320A/D1709A
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111631-plasmid-map-sequence-id-217672

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  19. pUPD1:Rb7-MAR

    Plasmid
    #106212
    Purpose
    Provides N.tabacum RB7 matrix attachment region as a level 0 GoldenBraid part
    Depositor
    Tomáš Moravec
    Insert
    N.tabacum RB7 matrix attachment region
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    106212-plasmid-map-sequence-id-205304

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Showing: 7821 - 7840 of 18384 results