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We narrowed to 18,351 results for: multi

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Showing: 7821 - 7840 of 18351 results

  3. pFastBacDual with LFn + FlaAAA

    Plasmid
    #84867
    Purpose
    Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.
    Depositor
    Russell Vance
    Publication
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    FlaAAA
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Mutation
    3 C-terminal leucines have been replaced by alani…
    Promoter
    Polyhedrin
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    84867-plasmid-map-sequence-id-162897

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  4. p3E-EGFP-NS-MCS

    Plasmid
    #75180
    Purpose
    EGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-NS-MCS
    Use
    Three prime entry vector for multisite gateway th…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75180-plasmid-map-sequence-id-153030

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  6. pU6_(GLuc)_TOP1

    Plasmid
    #68423
    Purpose
    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOP1 construct
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68423-plasmid-map-sequence-id-125554

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  7. RhoC FLARE.sc mCer, mVenus - CA

    Plasmid
    #65424
    Purpose
    RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L
    Depositor
    Klaus Hahn
    Publication
    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RBD, mCerulean, mVenus, RhoC
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    T153M in mVenus, Q63L in RhoC
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  8. RhoC FLARE.sc mCer, mVenus - DN

    Plasmid
    #65423
    Purpose
    RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N
    Depositor
    Klaus Hahn
    Publication
    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RBD, mCerulean, mVenus, RhoC
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    T153M in mVenus, T19N in RhoC
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. CCR8-DuET

    Plasmid
    #213204
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR8 (CCR8 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213204-plasmid-map-sequence-id-430767

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  17. pLJM1-B-GRAM-H-NeoR

    Plasmid
    #211709
    Purpose
    Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H)
    Depositor
    Yasunori Saheki
    Publication
    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:
    Insert
    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    Use
    Lentiviral
    Tags
    ddFP-B3
    Expression
    Mammalian
    Mutation
    Changed Glycine 187 to Leucine
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211709-plasmid-map-sequence-id-419206

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  18. pcDNA3.1_GEM7-Halo-FRB

    Plasmid
    #197058
    Purpose
    Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells
    Depositor
    Julia Mahamid
    Publication
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Encapsulin
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197058-plasmid-map-sequence-id-402600

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  19. pBFC0687

    Plasmid
    #186692
    Purpose
    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Publication
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_2xBsaI_NT (Guide Stuffer) gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    Expression
    Bacterial
    Promoter
    PLac
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186692-plasmid-map-sequence-id-371384

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Showing: 7821 - 7840 of 18351 results