We narrowed to 15,358 results for: nts

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pcDNA_DiCre_60.F2

Plasmid

#60208

Purpose

Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Human, Bacteriophage P1, Synthetic)

Tags

FRAP and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 2021-2112 of FRAP with a T2098L mutat…

Promoter

CMV

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf107

Plasmid

#12795

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf107

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
plc242-GFP-ITFG2

Plasmid

#87049

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Epsin 2

Plasmid

#22241

Depositor

Pietro De Camilli

Article

Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

Insert

Epsin 2 (EPN2 Human)

Tags

HA

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
ZIKV_Trigger_32B

Plasmid

#75009

Purpose

Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_Trigger_32B

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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Venus-7aa-ActA-pEGFP-C1

Plasmid

#177405

Purpose

To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

Depositor

David Andrews

Article

Osterlund et al Protocol Exchange (2022)

Insert

ActA tail anchor (actA Listeria monocytogenes)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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mKO-Mito-7

Plasmid

#57848

Purpose

Localization: Mitochondria, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Mito (COX8A Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-T308A

Plasmid

#49189

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

T308A

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only
pC1300_pUB10_pco-vCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

Plasmid

#197979

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-vCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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tdp43-EGFP construct11

Plasmid

#28204

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa193-274 only

Available Since

Sept. 8, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-6P-2_dCrk

Plasmid

#131146

Purpose

Expresses GST::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

GST

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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CoV2-N-D3L

Plasmid

#177945

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (D3L) (N )

Expression

Mammalian

Mutation

D3L

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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VQR variant

Plasmid

#138560

Purpose

Expresses human codon-optimized SpCas9-VQR and blasticidin resistance: EFS promoter-VQR-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

VQR

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, T1337R

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pFAB3833

Plasmid

#47839

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 8 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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CoV2-N-A119S

Plasmid

#177948

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (A119S) (N )

Expression

Mammalian

Mutation

A119S

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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SPLINTR BFP v1 backbone

Plasmid

#179779

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-eYFP-3x-miR708-5p-TS

Plasmid

#117381

Purpose

An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

EYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T2 SHP2 E76A

Plasmid

#8324

Depositor

Ben Neel

Article

O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

Insert

SHP2 (PTPN11 Human)

Tags

GST

Expression

Bacterial

Mutation

E76A (activated mutant)

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only
pRK5-MYC-ITFG2

Plasmid

#87046

Purpose

mammalian expression of ITFG2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

ITFG2 (ITFG2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pFAB3737

Plasmid

#47838

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 7 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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