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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 20,663 results for: LEA

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Showing: 7841 - 7860 of 20663 results

  1. Polymerase expression - pCMV-Phi29 (LM2708)

    Plasmid
    #208965
    Purpose
    Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Phi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Phi29(-exo;D169A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208965-plasmid-map-sequence-id-415813

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  3. PAGFP_NS2B3

    Plasmid
    #195755
    Purpose
    Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.
    Depositor
    Partha Mondal
    Publication
    Varghese et al bioRxiv 2022.12.18.520514
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dengue (Type-2) NS2B3
    Tags
    PA-GFP
    Expression
    Mammalian
    Mutation
    No mutations
    Promoter
    CMV
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    195755-plasmid-map-sequence-id-384111

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  5. HA-GFP1-10-actin-pcDNA4-TO

    Plasmid
    #118370
    Purpose
    Bimolecular Fluorescence Complimentation (BiFC) assay. Mammalian expression vector to generate stable, inducible cell line expressing N-terminally tagged HA-GFP1-10-actin.
    Depositor
    Maria Vartiainen
    Publication
    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    actin beta (ACTB Human)
    Tags
    HA-GFP1-10
    Expression
    Mammalian
    Promoter
    CMV/TO
    Available since
    May 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118370-plasmid-map-sequence-id-229456

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  6. pCEP4-Q842gp160

    Plasmid
    #100931
    Purpose
    Mammalian expression plasmid for Env from the Q842.d12 HIV-1 strain
    Depositor
    Erik Procko
    Publication
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HIV-1 (Q842.d12) Env
    Tags
    CD5 leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    100931-plasmid-map-sequence-id-202601

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  8. pcDNA3-RAB-AKARev(T/A)

    Plasmid
    #118479
    Purpose
    Negative-control mutant for RAB-AKARev biosensor.
    Depositor
    Jin Zhang
    Publication
    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAB-AKARev(T/A)
    Tags
    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…
    Expression
    Mammalian
    Mutation
    Contains Thr-to-Ala mutation in substrate sequenc…
    Promoter
    CMV
    Available since
    Dec. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118479-plasmid-map-sequence-id-230370

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  9. BC-Sig-GPRC

    Plasmid
    #44201
    Depositor
    Lei Xu
    Insert
    human GPR56 C-terminal fragment with signal peptide (ADGRG1 Human)
    Use
    Retroviral
    Tags
    none
    Expression
    Mammalian
    Mutation
    The C-terminal fragment of GPR56, starting at am…
    Promoter
    LTR
    Available since
    July 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    44201-plasmid-map-sequence-id-60060

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  14. DinoIII

    Plasmid
    #128100
    Purpose
    Dinoflagellate expression vector backbone. XbaI and BglII restriction sites for gene insertion.
    Depositor
    Senjie Lin
    Publication
    Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m
    Insert
    RNA complex & upstream/downstream of LHC
    Expression
    Bacterial
    Promoter
    Inserts are potential promoters
    Available since
    April 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    128100-plasmid-map-sequence-id-292693

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  16. pGEX6P1-DEST-HA

    Plasmid
    #119751
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal HA tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and HA
    Expression
    Bacterial
    Promoter
    tac promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    119751-plasmid-map-sequence-id-234027

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  17. Nemato

    Plasmid
    #24006
    Depositor
    Lawrence Cohen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VSFP
    Tags
    Cerulean and Citruline
    Expression
    Mammalian
    Available since
    April 2, 2010
    Availability
    Academic Institutions and Nonprofits only
    24006-plasmid-map-sequence-id-11277

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Showing: 7841 - 7860 of 20663 results