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pCIneo-lambdaN-HA-HsRCK-RFQNRtoEDAAA_AC

Plasmid

#148429

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA (DDX6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmMe31B-E208A-dsRNAres_AC

Plasmid

#148388

Purpose

Insect Expression of DmMe31B-E208A-dsRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmMe31B-E208A-dsRNAres (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G compared to the sequen…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmMe31B-K108A-dsRNAres_AC

Plasmid

#148389

Purpose

Insect Expression of DmMe31B-K108A-dsRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmMe31B-K108A-dsRNAres (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G compared to the sequen…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmMe31B-QHTKRKKtoAAAAAAA-dsRNAres_AC

Plasmid

#148390

Purpose

Insect Expression of DmMe31B-QHTKRKKtoAAAAAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmMe31B-QHTKRKKtoAAAAAAA-dsRNAres (me31B Fly)

Expression

Insect

Mutation

one silent mutation A1101G compared to the sequen…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF1_1-177_AE

Plasmid

#148570

Purpose

Mammalian Expression of HsGIGYF1_1-177

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_1-177 (GIGYF1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF1-Y39AY41AM46AL47A_AE

Plasmid

#148576

Purpose

Mammalian Expression of HsGIGYF1-Y39AY41AM46AL47A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1-Y39AY41AM46AL47A (GIGYF1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-HsGIGYF1_1-177-LFVENtoDDDAF_AE

Plasmid

#148581

Purpose

Mammalian Expression of HsGIGYF1_1-177-LFVENtoDDDAF

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_1-177-LFVENtoDDDAF (GIGYF1 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-E236Q-shRNAres_AC

Plasmid

#148450

Purpose

Mammalian Expression of HSRck-E236Q-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HSRck-E236Q-shRNAres (DDX6 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-K135A-shRNAres_AC

Plasmid

#148451

Purpose

Mammalian Expression of HsRck-K135A-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-K135A-shRNAres (DDX6 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-QHTRRKKtoAAAAAAA-shRNAres_AC

Plasmid

#148452

Purpose

Mammalian Expression of HsRck-QHTRRKKtoAAAAAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-QHTRRKKtoAAAAAAA-shRNAres (DDX6 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-QHTRtoAAAA-shRNAres_AC

Plasmid

#148453

Purpose

Mammalian Expression of HsRck-QHTRtoAAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-QHTRtoAAAA-shRNAres (DDX6 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-RFQNRtoEDAAA-shRNAres_AC

Plasmid

#148454

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA-shRNAres (DDX6 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Gln-tRNA-gRNA scaffold for library 1&2

Plasmid

#192367

Purpose

tRNA sequence provider (for checkpoint and immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gln tRNA-gRNA scaffold

Use

For golden gate assembly

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-62-SFFV-T2A-PuroR-polyA-R11b

Plasmid

#179893

Purpose

Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Expression

Bacterial and Mammalian

Promoter

SFFV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-64-SFFV-T2A-BlaR-polyA-R11b

Plasmid

#179895

Purpose

Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Expression

Bacterial and Mammalian

Promoter

SFFV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC

Plasmid

#148402

Purpose

Insect Expression of DmNot1_1674-1962-dsRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1_1674-1962-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

2 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR207-MUM2sp-ADPG2

Plasmid

#170723

Purpose

Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

Depositor

George Haughn

Article

McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

Insert

ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)

Use

Gateway donor vector / entry clone

Available Since

Nov. 11, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-KS-attB1-2-PT-SA-SD-0-mTagBFP2(11x7)

Plasmid

#172064

Purpose

Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 0 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

mTagBFP2(11x7)

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcryGFP-acta1-dmdGFP

Plasmid

#172530

Purpose

alphaA-crystallin promoter (cry) drives GFP expression in the lens. The acta1 muscle-specific promoter drives expression of GFP-tagged zebrafish dmd.

Depositor

Joachim Berger

Article

Berger et al J Cell Mol Med. 2020 Jun;24(12):6680-668

Insert

dmd (dmd Zebrafish)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmpr2 gRNA resistant

Plasmid

#163408

Purpose

Expresses BMPR-2 resistant to the gRNAs(#163388-90) in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmpr2 (Bmpr2 Mouse)

Expression

Mammalian

Mutation

gRNA-targeting sequences are substituted with nuc…

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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