We narrowed to 19,051 results for: Tec

1. pCMV-Target-ACEmax

   Plasmid

   #139105

   Purpose

   Mammalian Target-ACEmax expression vector (pSI-625)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Td-CBEmax

   Plasmid

   #196600

   Purpose

   Mammalian expression of Td-CBEmax

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L/E27R)-32aa linker-nSpCas9-bpNLS-P2A-UGI-bpNLS
   

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   TadA-8e (N46L/E27R); S. pyogenes Cas9 (D10A)

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDY1462_SERPINA1_CCA32_Caspase

   Plasmid

   #193194

   Purpose

   Express Caspase conditional upon RNA transcript

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

   Insert

   iCaspase9 (CASP9 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-B-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207377

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ROS lux sensor

   Plasmid

   #199791

   Purpose

   Synthetic gene circuit

   Depositor

   Timothy Lu

   Article

   Inda-Webb et al Nature. 2023 Aug;620(7973):386-392. doi:

   Insert

   ROS lux Sensor
   

   Use

   Synthetic Biology

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFP5380 LAMP1-SBP- mCherry-FIREtag

   Plasmid

   #216725

   Purpose

   Expresses LAMP1-mCherry-FIREtag in mamallian cells

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   LAMP-1 (LAMP1 Human)
   

   Tags

   FIREtag, Streptavidin Binding Protein (SBP), and …

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. nCas9-PPE

   Plasmid

   #140445

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   nCas9(H840A)-M-MLV
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   H840A for Cas9; D200N, T306K, W313F, T330P and L…

   Promoter

   maize Ubiquitin-1

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPapi

   Plasmid

   #96921

   Purpose

   expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.

   Depositor

   John Doench, David Root

   Article

   Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. eTd-CBE

   Plasmid

   #196601

   Purpose

   Mammalian expression of eTd-CBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

   Insert

   bpNLS-TadA-8e (N46L)-nSpCas9-bpNLS-P2A-UGI-bpNLS
   

   Tags

   6*His

   Expression

   Mammalian

   Available Since

   March 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TccCas13a Twin SUMO

    Plasmid

    #199754

    Purpose

    Expresses codon optimized thermostabile TccCas13a in E.coli

    Depositor

    Magdy Mahfouz

    Article

    Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    TccCas13a
    

    Tags

    SUMO

    Expression

    Bacterial

    Promoter

    LacI

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. ABE8.17-m

    Plasmid

    #136298

    Purpose

    expresses ABE8.17-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.17-m
    

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2T-cmv-SpCas9-TadCBEd-BlastR

    Plasmid

    #193848

    Purpose

    Express TadCBEd in mammalian cells with BlastR selection, Tol2 integration

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadCBEd
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Gβ-2A-cpV-Gγ2-IRES-Gαi2-mTq2

    Plasmid

    #69624

    Purpose

    A FRET sensor for Galphai2 activation, encoded on a single plasmid.

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Inserts

    Gbeta-2A-cpV-Ggamma2
    Galphai2-mTurquoise2
    

    Tags

    ciruclar permutated Venus (cp173V) and mTurquoise…

    Expression

    Mammalian

    Promoter

    CMV and CMV (IRES)

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmCherry-HP1alpha

    Plasmid

    #179703

    Purpose

    visualization of centromere heterochromatin

    Depositor

    Kenji Sugimoto

    Article

    Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

    Insert

    heterochromatin protein alpha (CBX5 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-sgRNA-lib 2.0

    Plasmid

    #89638

    Purpose

    sgRNA expression in mammalian cells after Gateway cloning

    Depositor

    Wensheng Wei

    Article

    Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PET-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207374

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. scaffold-linker-U6

    Plasmid

    #89639

    Purpose

    construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

    Depositor

    Wensheng Wei

    Article

    Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9-NRCH-YE1-BE4max

    Plasmid

    #198549

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-YE1-BE4max
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pL-CRISPR.SFFV.tRFP

    Plasmid

    #57826

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-tRFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pv9_TAF3_2xPHD-13XL-BASU

    Plasmid

    #179414

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of TAF3.2xPHD-13XL-BASU

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    TAF3.2xPHD-13XL-BASU
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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