We narrowed to 11,619 results for: VARS

1. pαH-S-GSAS-OMICRON.BA.2-RBD

   Plasmid

   #184830

   Purpose

   Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

   Depositor

   Priyamvada Acharya

   Article

   Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

   Insert

   S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   6X His tag

   Expression

   Mammalian

   Mutation

   SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

   Available Since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_CHUK_WT

   Plasmid

   #82083

   Purpose

   Gateway Donor vector containing CHUK , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   CHUK (CHUK Human)
   

   Use

   Gateway entry vector

   Mutation

   V268I

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-N 16E7

   Plasmid

   #37881

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   HPV 16 E7 (E7 HPV)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   July 26, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pDONR223_FBXW7_p.R505C

   Plasmid

   #81507

   Purpose

   Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   FBXW7 (FBXW7 Human)
   

   Use

   Gateway entry vector

   Mutation

   R505C

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-KrasG12C/sgKras/Cre

   Plasmid

   #99850

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G12C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223_SMARCB1_WT

   Plasmid

   #81743

   Purpose

   Gateway Donor vector containing SMARCB1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SMARCB1 (SMARCB1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_HRAS_p.G12D

   Plasmid

   #81284

   Purpose

   Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, David Root, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   HRAS (HRAS Human)
   

   Use

   Gateway entry vector

   Mutation

   G12D

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_CMVTRE3G_LYSET-Isoform2_Flag

   Plasmid

   #191999

   Purpose

   Lentiviral vector to generate flag-tagged LYSET(TMEM251)-isoform2 stable expressing cell line under CMVTRE3G promoter

   Depositor

   Jan Carette

   Article

   Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

   Insert

   LYSET-Isoform2 (LYSET Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMVTRE3G

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti_CMVTRE3G_LYSET-Isoform1_untagged-Puro

   Plasmid

   #192001

   Purpose

   Lentiviral vector to generate LYSET(TMEM251)-isoform1 stable expressing cell line under CMVTRE3G promoter

   Depositor

   Jan Carette

   Article

   Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

   Insert

   LYSET-Isoform1 (LYSET Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMVTRE3G

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid

    #114082

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    

    Tags

    2x SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-KrasG12V/sgKras/Cre

    Plasmid

    #99854

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12V mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-N BARF1

    Plasmid

    #37922

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    BARF1 (BARF1 H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pαH-S-GSAS-OMICRON.BA.1-RBD

    Plasmid

    #184831

    Purpose

    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

    Insert

    S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    6X His tag

    Expression

    Mammalian

    Mutation

    SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR223_CXCR4_WT

    Plasmid

    #81957

    Purpose

    Gateway Donor vector containing CXCR4, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CXCR4 (CXCR4 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSCV-C 16E6

    Plasmid

    #37876

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    HPV 16E6 (E6 HPV)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    July 25, 2012

    Availability

    Academic Institutions and Nonprofits only

16. Ai140 (TIT2L-GFP-ICL-tTA2) targeting vector

    Plasmid

    #114427

    Purpose

    Target a Cre-dependent GFP and a tTA2 expression cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    GFP, tTA2
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Mammalian

    Promoter

    PGK, CAG

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. PIG-MycT58A

    Plasmid

    #177648

    Purpose

    Expression of mouse Myc with T58A point mutation

    Depositor

    Trudy Oliver

    Article

    Mollaoglu et al Cancer Cell. 2017 Feb 13;31(2):270-285.

    Insert

    Myc-T58A (Myc Mouse)
    

    Use

    Retroviral

    Tags

    GFP and IRES

    Expression

    Mammalian

    Mutation

    T58A point mutation

    Available Since

    Feb. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ai133 (TITL-ssAPEX2tm) targeting vector

    Plasmid

    #114432

    Purpose

    Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    ssAPEX2tm
    

    Use

    Cre/Lox and Mouse Targeting

    Promoter

    TREtight

    Available Since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR D-TOPO-C3_33

    Plasmid

    #60275

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    GLIS3 enhancer (GLIS3 Human)
    

    Use

    Entry vector

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

    Plasmid

    #114081

    Purpose

    CAG promoter expression plasmid for human codon optimized enAsBE1.1

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    E174R, S542R, K548R and D908A, human codon optimi…

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge