We narrowed to 10,238 results for: ada

1. shERK2b-mlpx-puro

   Plasmid

   #65230

   Purpose

   encodes a shRNA against ERK2

   Depositor

   Gerardo Ferbeyre

   Article

   Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

   Insert

   shRNA against ERK2 (MAPK1 Human)
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA4-deltaH-AMBRA1-3xFLAG

   Plasmid

   #174158

   Purpose

   Vector for expression of 3xFLAG-tagged truncated AMBRA1 that cannot bind DDB1 (aa 1-34 deleted)

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA1 (AMBRA1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Missing aa 1-34

   Promoter

   CMV

   Available Since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1 KBTBD4 P311PP

   Plasmid

   #184625

   Purpose

   Doxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 P311PP expression in mammalian cells

   Depositor

   Vincenzo D'Angiolella

   Article

   Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

   Insert

   N-terminal Flag tagged KBTBD4 P311PP (KBTBD4 Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Mutation

   P311PP

   Promoter

   TRE promoter, Tet ON

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-mTHC

   Plasmid

   #112620

   Purpose

   expression plasmid with CAG promoter driving multicistronic cassette H2BmTeal_p2A_HygroR_p2A_CreERt2

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-mTFP1 (H2BC3 Anthozoa, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Tags

   mTeal (mTFP1)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRetroX-TetOne-Puro-p62 deltaUBA

   Plasmid

   #204550

   Purpose

   Expresses p62 deltaUBA in a doxycycline-dependent manner in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   p62 (SQSTM1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   deltaUBA

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-KLF6

   Plasmid

   #192909

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of KLF6

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   KLF6 (KLF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.23 C3_7

   Plasmid

   #60293

   Purpose

   This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

   Depositor

   Jorge Ferrer

   Article

   Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

   Insert

   SLC30A8 enhancer (SLC30A8 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pEN-L1-K2-L2

   Plasmid

   #80684

   Purpose

   Gateway Entry vector containing a temperature-sensitive mouse DHFR

   Depositor

   Nico Dissmeyer

   Article

   Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

   Inserts

   DHFR (Dhfr Mouse)
   DHFR (Dhfr Mustard Weed, Mouse)
   

   Use

   Synthetic Biology

   Tags

   3xHA

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   mouse DHFR with N-terminal Ubiquitin

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVHC_PGKneoLox2DTA.2

   Plasmid

   #112622

   Purpose

   H2BVenus_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

   Depositor

   Stefan Heller

   Article

   Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

   Inserts

   H2B-Venus (H2BC3 Aequorea victoria, Human)
   Hygrophosphotransferase
   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox and Mouse Targeting

   Tags

   Venus

   Promoter

   No Promoter

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 KBTBD4 P311PP

    Plasmid

    #184628

    Purpose

    Mammalian expression vector with N-terminal 3xFlag for KBTBD4 P311PP expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 P311PP (KBTBD4 Human)
    

    Expression

    Mammalian

    Mutation

    P311PP

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA 3.1 KBTBD4 R312RG

    Plasmid

    #184630

    Purpose

    Mammalian expression vector with N-terminal Flag for KBTBD4 R312RG expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 R312RG (KBTBD4 Human)
    

    Expression

    Mammalian

    Mutation

    R312RG

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. shERK2a-mlpx-puro

    Plasmid

    #65229

    Purpose

    encodes a shRNA against ERK2

    Depositor

    Gerardo Ferbeyre

    Article

    Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

    Insert

    shRNA against ERK2 (MAPK1 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQC hGAB.wt V5-His IRES G418

    Plasmid

    #110333

    Purpose

    Mammalian retroviral expression of glutaminase 2 isoform GAB (wild type) with V5 and 6xHis tags

    Depositor

    Sandra Martha Gomes Dias

    Article

    Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

    Insert

    GLS2 Glutaminase 2 (GLS2 Human)
    

    Use

    Retroviral

    Tags

    6xHis and V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid

    #105801

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: FLAG-3C; ORF2: mClover3-TEV

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVRC8400-SARS-CoV-2-S2P-B.1.617.2-AVI

    Plasmid

    #176334

    Purpose

    Expresses SARS-CoV-2 S2P protein from the B.1.617.2 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Article

    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

    Insert

    SARS-CoV-2-S2P-B.1.617.2 (S )
    

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19R, G142D, del156-157, R158G, L452R, T478K, D61…

    Available Since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSin-EF2-DMD53-GFP

    Plasmid

    #138353

    Purpose

    Expressing a GFP reporter cassette, whose GFP reading frame is disrupted by insertion of partial DMD exon 53 sequences

    Depositor

    Baisong Lu

    Article

    Lyu et al PLoS One. 2020 Sep 24;15(9):e0239468. do

    Insert

    DMD exon 53 partial sequence to disrupt GFP reading frame (DMD Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Promoter

    EF2

    Available Since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_SEPT2 NCmut-msfGFP

    Plasmid

    #180320

    Purpose

    mammalian expression of human SEPT2 NCmut fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    SEPT2 F20D, V27D

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Venus

    Plasmid

    #105805

    Purpose

    Concomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Venus, cleavable by TEV. Useful for protein interaction analysis by FRET.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: 3C-Cerulean; ORF2: TEV-Venus

    Expression

    Mammalian

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKHH030_sgRB1#1

    Plasmid

    #174144

    Purpose

    Lentiviral vector expressing a sgRNA against the human RB1 gene

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    RB1 (RB1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.puro_shGLS

    Plasmid

    #110335

    Purpose

    shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Article

    Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

    Insert

    GLS glutaminase (GLS Synthetic, Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA PolIII)

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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