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pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

Plasmid

#213524

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to S

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

Plasmid

#213525

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed P335 to H

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

Plasmid

#213526

Purpose

Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

Depositor

Thomas Tuschl

Article

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)

Tags

His6-sumo

Expression

Bacterial

Mutation

changed H424 to R

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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2M-T-Mce3R_R53A

Plasmid

#225727

Purpose

To express Mce3R mutant R53A in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis-MBP

Expression

Bacterial

Mutation

R53A

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-TrxC

Plasmid

#225742

Purpose

To express TrxC(Rv3914) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

TrxC

Tags

6xHis

Expression

Bacterial

Mutation

TrxC

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-TrxB2

Plasmid

#225741

Purpose

To express TrxB2(Rv3913) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

TrxB2

Tags

6xHis

Expression

Bacterial

Mutation

TrxB2

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-M1

Plasmid

#225734

Purpose

To express M1 (13-193) in E. coli

Depositor

Nicole Sampson

Article

Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

Insert

mce3R

Tags

6xHis

Expression

Bacterial

Mutation

aa 13-193

Available Since

Oct. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF_ApyA

Plasmid

#226249

Purpose

Production of unmodified ApyA peptide.

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Insert

His6_ApyA

Tags

Hexa-Histidine

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-IRSp53 P428L

Plasmid

#221321

Purpose

Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 (Baiap2 Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

P428L

Promoter

TRE3G

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-GMIP P564D

Plasmid

#221324

Purpose

Dox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

P564D

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-GMIP S31D

Plasmid

#221322

Purpose

Dox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

S31D

Promoter

TRE3G

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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plx208-CMV-SspB-EGFP-VP16

Plasmid

#213532

Purpose

Expresses one of the split transcription factor component in mammalian cells

Depositor

Wenjing Wang

Article

Shen et al Nat Methods. 2023 Jan;20(1):112-122. doi

Insert

SspB-EGFP-VP16

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEG302-TRB1-ZF

Plasmid

#200925

Purpose

Express TRB1-ZF108 in Arabidopsis target FWA gene

Depositor

Steven Jacobsen

Article

Wang et al Nat Commun. 2023 Mar 28;14(1):1736. doi:

Insert

TRB1 (TRB1 Mustard Weed)

Expression

Plant

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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hPRL3_1-169_C104D

Plasmid

#209797

Purpose

Bacterial expression of human PRL-3 C104D mutant without farnesylation motif

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2020 Aug 14;295(33):11682-1

Insert

PRL-3 (PTP4A3 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 1-169 only; C101D

Promoter

T7

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Shim_CytC_TP_GFP_pK7FWG2

Plasmid

#202646

Purpose

Determine where transit peptide targets GFP in N. benthamiana

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from putative Cytochrome C

Tags

Green Florescent Protien

Expression

Plant

Available Since

Aug. 10, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAG416GALL-SARS-CoV-2_3CL

Plasmid

#203496

Purpose

Expresses SARS-CoV-2 3CL protease from a GALL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SARS-CoV-2 3CL protease (ORF1ab )

Expression

Yeast

Promoter

GALL

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIH FRB Ubiqutin-BFP

Plasmid

#202426

Purpose

TagBFP FKBP-rapamycin binding (FRB) dimerization domain fused to ubiquitin

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

FKBP-rapamycin binding (FRB) dimerization domain

Use

Lentiviral

Tags

Ubiquitin, TagBFP

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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vYM004_pNeo_phiC31_nTRE_pEF1s(orig)_citrine

Plasmid

#197677

Purpose

"pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsPatL1-del399-516_J

Plasmid

#146668

Purpose

Mammalian Expression of HsPatL1-del399-516

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1-del399-516 (PATL1 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuetP-HsPatL1_517-770-Mut1_J

Plasmid

#146672

Purpose

Bacterial Expression of HsPatL1_517-770-Mut1

Depositor

Elisa Izaurralde

Article

Braun et al EMBO J. 2010 Jul 21. 29(14):2368-80.

Insert

HsPatL1_517-770-Mut1 (PATL1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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