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pBY011-AMN1ko

Plasmid

#183099

Purpose

S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.

Depositor

Gabor Balazsi

Article

Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:

Insert

AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)

Tags

KanMX

Expression

Bacterial and Yeast

Mutation

only includes external sequences (504 bp each) as…

Promoter

URA3, TEF1

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-anti-MIR328-3p

Plasmid

#153319

Purpose

Expresses zsGreen fused to a miR-328-3p sponge and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen with MIR328-3p sponge

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-anti-MIR211-5p

Plasmid

#153318

Purpose

Expresses zsGreen fused to a miR-211-5p sponge and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

zsGreen with MIR211-5p sponge

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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p23-mRuby3-DDX21 HGV

Plasmid

#179233

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Tags

mRuby3

Expression

Mammalian

Mutation

D338H, E339G

Promoter

EF-1alpha

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmAGO2-RC_B

Plasmid

#145980

Purpose

Insect Expression of DmAGO2-RC

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

DmAGO2-RC (AGO2 Fly)

Expression

Insect

Mutation

A deletion of AA 51-56 and an insertion of 23 AA …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.4.hSyn.flex.H2B.RFP

Plasmid

#170386

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 4. Cre-inducible expression of nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre inducible H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.3.hSyn.flex.H2B.RFP

Plasmid

#170374

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 3. Cre-inducible expression of nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre inducible H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gT28.13.hSyn.flex.H2B.RFP

Plasmid

#170376

Purpose

Plasmid used for Crispr/cas9 based disruption of mouse Trim28, guide targeting exon 13. Cre-inducible expression of nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre inducible H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCB’ CsoS2 ΔNTD

Plasmid

#162705

Purpose

Carboxysome operon with truncated CsoS2 (deletion of amino acids 2-256), prk; Deletion of rubisco-binding N-terminus of CsoS2

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Expression

Bacterial

Mutation

Deletion of residues 2-256 of CsoS2, Y131D

Promoter

PLtet0-1 promoter

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-GST-PolB(K206A/K244A)

Plasmid

#177135

Purpose

Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A) with a TEV protease site located between the GST tag and PolB(K206A/K244A)

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB

Tags

GST

Expression

Bacterial

Promoter

Tac

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42 -SSRP1

Plasmid

#170439

Purpose

Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

Depositor

Joan Conaway

Article

Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

Insert

ssrp1 (Ssrp Fly)

Tags

6xHis and V5

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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R9-FS1

Plasmid

#125208

Purpose

Expressing human Rad9b frameshift mutation found in a neural tube defect patient

Depositor

Richard Finnell

Article

Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

Insert

Rad9b (RAD9B Human)

Expression

Mammalian

Mutation

p.S318SfsX28

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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R9-FS2

Plasmid

#125209

Purpose

Expressing human Rad9b frameshift mutation found in a neural tube defect patient

Depositor

Richard Finnell

Article

Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

Insert

Rad9b (RAD9B Human)

Expression

Mammalian

Mutation

p.A401Rfs4X

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 delta787-843

Plasmid

#166821

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

_787-843

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 Ub-NLRP1 S1213 P1214R

Plasmid

#166830

Purpose

pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Tags

Ubiquitin

Mutation

P1214R

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 T755N

Plasmid

#166820

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

T755N

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 R726W

Plasmid

#166819

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

R726W

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R459E

Plasmid

#164024

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R459E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C R464E

Plasmid

#164025

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, R464E

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 CARD A452C E485R

Plasmid

#164026

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD (filment-deficient mutant and A452C mutation for cysteine labelling applications)

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

A452C, E485R

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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