We narrowed to 11,619 results for: VARS

1. pAAV-U6sgp53-mTSG-GFAPCre

   Plasmid

   #100276

   Purpose

   AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

   Insert

   GFAP promoter (Gfap, GFAP Human, Mouse)
   

   Use

   AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

   Expression

   Mammalian

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_HRAS_p.Q61R

   Plasmid

   #81311

   Purpose

   Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   HRAS (HRAS Human)
   

   Use

   Gateway entry vector

   Mutation

   Q61R

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_MAP2K4_WT

   Plasmid

   #82142

   Purpose

   Gateway Donor vector containing MAP2K4 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   MAP2K4 (MAP2K4 Human)
   

   Use

   Gateway entry vector

   Mutation

   P27R; p.8delG

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_AKT3_WT

   Plasmid

   #82093

   Purpose

   Gateway Donor vector containing AKT3 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   AKT3 (AKT3 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ai161 (TIT2L-GFP-ICR-tTA2) targeting vector

   Plasmid

   #114429

   Purpose

   Target a Cre-dependent GFP cassette and Dre-dependent tTA2 cassette to the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GFP
   

   Use

   Cre/Lox and Mouse Targeting

   Promoter

   TRE2, CAG

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter

   Plasmid

   #172596

   Purpose

   Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.

   Depositor

   Daniel Kiss

   Article

   Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2

   Insert

   myc-PDGFRA-R914W (PDGFRA Human, Synthetic)
   

   Use

   Egfp-pest_reporter

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   PDGFRA-R914W

   Promoter

   CMV

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_IDH2_p.R172M

   Plasmid

   #81618

   Purpose

   Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   IDH2 (IDH2 Human)
   

   Use

   Gateway entry vector

   Mutation

   R172M

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-C GFP

   Plasmid

   #37856

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   GFP
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pDONR223_KRAS_p.D33E

   Plasmid

   #81659

   Purpose

   Gateway Donor vector containing KRAS,part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry vector

   Mutation

   D33E

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDONR223_AKT1_p.Q79K

    Plasmid

    #81479

    Purpose

    Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Gateway entry vector

    Mutation

    Q79K

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_MAPK6_p.D387N

    Plasmid

    #81712

    Purpose

    Gateway Donor vector containing MAPK6, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    MAPK6 (MAPK6 Human)
    

    Use

    Gateway entry vector

    Mutation

    L290V; D387N; del amino acids 1-8

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-KrasG12R/sgKras/Cre

    Plasmid

    #99852

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-28b-T7-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1931)

    Plasmid

    #114075

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R) with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    E174R, S542R, K548V and N552R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-28b-T7-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1902)

    Plasmid

    #114077

    Purpose

    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) with C-terminal NLS and His-tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized AsCas12a-enRR (E174R/S542R/K607R) with C-terminal NLS and His-tag
    

    Tags

    NLS(nucleoplasmin) and 6xHis

    Expression

    Bacterial

    Mutation

    E174R/S542R/K607R

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_IKBKG_WT

    Plasmid

    #82242

    Purpose

    Gateway Donor vector containing IKBKG , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    IKBKG (IKBKG Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_RASA1_p.L146S

    Plasmid

    #81606

    Purpose

    Gateway Donor vector containing RASA1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RASA1 (RASA1 Human)
    

    Use

    Gateway entry vector

    Mutation

    L146S

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223_HRAS_WT

    Plasmid

    #81938

    Purpose

    Gateway Donor vector containing HRAS, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV-C 6bE7

    Plasmid

    #37917

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    HPV 6b E7 (E7 HPV)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pGL4.23 C3_20

    Plasmid

    #60303

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    CDKN1C enhancer (CDKN1C Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. AAV-KrasG13D/sgKras/Cre

    Plasmid

    #99857

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge