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pACYCDuet1-ammA(∆Trp56)

Plasmid

#134683

Purpose

Expresses His6-AmmA(∆Trp56) in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammA(delW56)

Tags

His6

Expression

Bacterial

Mutation

deletion of W56

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-GFP-tglG

Plasmid

#134679

Purpose

Expresses His6-GFP-TglG fusion in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglG

Tags

His6-GFP

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b-tglHI

Plasmid

#134672

Purpose

Expresses His6-TglH and TglI in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Inserts

TglH
TglI

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-Rab27b

Plasmid

#109762

Purpose

Muscle specific zebrafish expression of mKate2 fused to Rab27b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab27b (rab27b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-Rab27b

Plasmid

#109754

Purpose

Multisite gateway vector for 3' tagging with Rab27b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rab27b (rab27b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab3ab

Plasmid

#109704

Purpose

Multisite gateway vector for 3' tagging with rab3ab

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab3ab (rab3ab Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab42b

Plasmid

#109711

Purpose

Multisite gateway vector for 3' tagging with rab42b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab42b (rab42b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab24

Plasmid

#109681

Purpose

Multisite gateway vector for 3' tagging with rab24

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab24 (rab24 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-rab25b

Plasmid

#109683

Purpose

Multisite gateway vector for 3' tagging with rab25b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab25b (rab25b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab42b

Plasmid

#109643

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab42b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab42b (rab42b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab24

Plasmid

#109613

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab24

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab24 (rab24 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab25b

Plasmid

#109615

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab25b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab25b (rab25b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDK540

Plasmid

#134201

Purpose

Bacterial expression of C. elegans KNL1

Depositor

Iain Cheeseman

Article

Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

Insert

KNL1 FL with YA mutant (knl-1 Nematode)

Expression

Bacterial

Mutation

YA

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

Plasmid

#133343

Purpose

for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

Plasmid

#133345

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_blaA

Use

CRISPR

Promoter

constitutive

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-Vmn1r85

Plasmid

#133414

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 85

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r78 (Vmn1r78 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP5g-P2A-Vmn1r89

Plasmid

#133416

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 89

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry-Vmn1r89 (Vmn1r89 Mouse)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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UL79(E48A_L49A)-3xFLAG pCDNA4

Plasmid

#129759

Purpose

Expresses CMV UL79 (E48A_L49A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

UL79 (UL79 Human Cytomegalovirus (HHV-5))

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glutamic acid 48 to Alanine and Leucine 4…

Promoter

CMV

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*R47W)

Plasmid

#125659

Purpose

pCMV-ABEmax(TadA E59A, TadA*R47W) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* R47W)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*R47M)

Plasmid

#125660

Purpose

pCMV-ABEmax(TadA E59A, TadA*R47M) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* R47M)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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