Addgene

We narrowed to 139 results for: ca3

Showing: 91 - 120 of 139 results

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pEGFP-C1-Ataxin3Q28

Plasmid

#22122

Depositor

Henry Paulson

Article

Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

Insert

Ataxin 3 (ATXN3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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pVETL-Cmcs-Flag-Ataxin3Q22-C14A

Plasmid

#22119

Depositor

Henry Paulson

Article

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C14A

Promoter

Available since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only
pVETL-Cmcs-Flag-Ataxin3Q80-C14A

Plasmid

#22121

Depositor

Henry Paulson

Article

Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

Insert

Ataxin 3 (ATXN3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C14A

Promoter

Available since

Nov. 10, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-6P1-Ataxin3Q22

Plasmid

#22117

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Ataxin3Q22-C14A

Plasmid

#22113

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C14A

Promoter

Available since

Sept. 16, 2009

Availability

Academic Institutions and Nonprofits only
pGEX-6P1-Ataxin3Q22-UIM*

Plasmid

#22114

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

UIM 1, 2, 3 SA-DG

Promoter

Available since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only
pLJC2-TK2-3xFLAG

Plasmid

#217427

Purpose

Lentiviral overexpression of human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

TK2 (TK2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert sequence is a codon-optimized gBLOCK (IDT)

Promoter

CMV

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTK2_4

Plasmid

#217431

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 4 targeting TK2 (TK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTK2_5

Plasmid

#217432

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 5 targeting TK2 (TK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM6-Flag

Plasmid

#180411

Purpose

lentiviral transduction of human TGM6 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM6 (TGM6 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RON-V5/HIS

Plasmid

#201983

Purpose

expression of human RON receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

RON receptor tyrosine kinase (MST1R Human)

Use

Tags

V5, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P1-Ataxin3-1-182

Plasmid

#22115

Depositor

Henry Paulson

Article

Winborn et al J Biol Chem. 2008 Sep 26. 283(39):26436-

Insert

Ataxin 3 (ATXN3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 183-373 deleted

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pInducer20 2xHA HCAR3

Plasmid

#226811

Purpose

Tet-inducible lentiviral vector for 2xHA HCAR3 expression

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

HCAR3 (HCAR3 Human)

Use

Lentiviral

Tags

2xHA tag followed by a GDPPVAT linker

Expression

Mutation

Sequence optimized from NP_006009.2

Promoter

Available since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA HCAR3

Plasmid

#226807

Purpose

Gateway entry vector for 2xHA HCAR3

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

HCAR3 (HCAR3 Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Expression

Mutation

Sequence optimized from NP_006009.2

Promoter

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3

Plasmid

#89975

Purpose

Expresses GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Clta KI

Plasmid

#131483

Purpose

Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Clta Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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MST1R gRNA (BRDN0001487060)

Plasmid

#78050

Purpose

3rd generation lentiviral gRNA plasmid targeting human MST1R

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MST1R (MST1R Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MST1R gRNA (BRDN0001145791)

Plasmid

#78051

Purpose

3rd generation lentiviral gRNA plasmid targeting human MST1R

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MST1R (MST1R Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MST1R gRNA (BRDN0001145169)

Plasmid

#78052

Purpose

3rd generation lentiviral gRNA plasmid targeting human MST1R

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MST1R (MST1R Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-ataxin-3 C14A

Plasmid

#89977

Purpose

Expresses catalytic inactive GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed Cysteine 14 to Alanine

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBig1a zz TEV YBBR TPP1 MBP TEV TIN2

Plasmid

#185446

Purpose

Coexpresses human TPP1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site and human TIN2 with an MBP affinity tag and TEV cleavage site in insect cells

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Inserts

TPP1 (TPP1 Human)
TIN2 (TINF2 Human)

Use

Tags

MBP, YBBR, and ZZ

Expression

Insect

Mutation

Promoter

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 N-t

Plasmid

#89980

Purpose

Expresses the GFP-tagged N-terminus (Josephin domain) of ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

construct contains aa 1-182; deleted aa 183-361

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 C-t

Plasmid

#89981

Purpose

Expresses the GFP-tagged C-terminus of ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-182

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
10xHis-ataxin-3 *SIM-HA

Plasmid

#89983

Purpose

Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

10xHis and HA

Expression

Mammalian

Mutation

mutated aa 162IFVV to 162AFAA

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-ataxin-3 *SIM

Plasmid

#89979

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutated 162IFVV to 162AFAA

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-ataxin-3 *VBM

Plasmid

#89976

Purpose

Expresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motif

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutated 282RKRR to 282HNHH

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TK2 gRNA (BRDN0001144837)

Plasmid

#77297

Purpose

3rd generation lentiviral gRNA plasmid targeting human TK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TK2 (TK2, TK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TK2 gRNA (BRDN0001146931)

Plasmid

#77298

Purpose

3rd generation lentiviral gRNA plasmid targeting human TK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TK2 (TK2, TK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TK2 gRNA (BRDN0001149097)

Plasmid

#77299

Purpose

3rd generation lentiviral gRNA plasmid targeting human TK2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TK2 (TK2, TK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4

Plasmid

#214137

Purpose

Insect cell expression of the human 20SCP proteasome assembly chaperones

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

POMP (POMP Human)
PSMG1 (PSMG1 Human)
PSMG2 (PSMG2 Human)
PSMG3 (PSMG3 Human)
PSMG4 (PSMG4 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge