Addgene

We narrowed to 3,722 results for: AAT

Showing: 61 - 80 of 3722 results

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pNH605-CCAAT-YFP

Plasmid

#172132

Purpose

Fluorescent reporter for HAP complex activity

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xCCAAT-YFP

Use

Vector for genomic integration into the yeast gen…

Tags

Expression

Yeast

Mutation

Promoter

CYC1 core promotor with 4 tandem repeats of the H…

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAGT8162 (AATG_dttLbCas12a-i_TTCG)

Plasmid

#192350

Purpose

L0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expression

Depositor

Tom Schreiber

Article

Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

Insert

AATG_dttLbCas12a-i_TTCG

Use

CRISPR

Tags

Expression

Mutation

D156R; E925Q

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pegAAT K342E correction

Plasmid

#169841

Purpose

Express a pegRNA used for correction (via A•T-to-G•C) of the E342K mutation

Depositor

Wen Xue

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

pegAAT K342E correction (SERPINA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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AATK gRNA (BRDN0001146583)

Plasmid

#77315

Purpose

3rd generation lentiviral gRNA plasmid targeting human AATK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AATK (AATK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AATK gRNA (BRDN0001147695)

Plasmid

#77316

Purpose

3rd generation lentiviral gRNA plasmid targeting human AATK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AATK (AATK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AATK gRNA (BRDN0001148887)

Plasmid

#77314

Purpose

3rd generation lentiviral gRNA plasmid targeting human AATK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

AATK (AATK Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-mouse Plaat3 (WT)

Plasmid

#167987

Purpose

Expresses EGFP-Plaat3 in mammalian cells

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Hrasls3 (Plaat3 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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AatII-pRPS5A-PTP-3Xflag

Plasmid

#189639

Purpose

sTALED for plants

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET11a-DAAT-V33G/T242G

Plasmid

#159278

Purpose

Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

Depositor

Roberto Chica

Article

Parmeggiani et al ACS Catalysis 9, 3482–3486.

Insert

Engineered d-alanine aminotransferase

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Valine 33 and threonine 242 have been mutated to …

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + M-AAT target

Plasmid

#86008

Purpose

lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
M-AAT target

Use

Lentiviral

Tags

Expression

Mutation

V30M

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + Z-AAT target

Plasmid

#86007

Purpose

lenti reporter plasmid with Z-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
Z-AAT target

Use

Lentiviral

Tags

Expression

Mutation

V30M

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-UBC-hAAT-IRES-ZsGreen-W

Plasmid

#126702

Purpose

lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

Depositor

Darrell Kotton

Article

Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

Insert

Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UBC

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-mouse Plaat3 (N157stop)

Plasmid

#168771

Purpose

Expresses EGFP-Plaat3 (C-terminal element is deleted) in mammalian cells

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Hrasls3 (Plaat3 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2-aACry::EGFP-zebrafish Plaat1 (WT)

Plasmid

#168772

Purpose

Express EGFP-zebrafish Plaat1 (WT) in zebrafish lens

Depositor

Noboru Mizushima

Article

Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

Insert

Hrasls (plaat1 Zebrafish)

Use

Tags

EGFP

Expression

Mutation

Promoter

alphaA-crystallin promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

Plasmid

#163030

Purpose

Expression of CRISPRi with gSTOP gRNA

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

AAV

Tags

KRAB, SV40 pA, and myc NLS

Expression

Mammalian

Mutation

Promoter

Alb-AAT

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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(564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9

Plasmid

#163025

Purpose

Expression of CRISPRi with gRNA against mouse Pcsk9

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9 (Pcsk9 S. aureus)

Use

AAV

Tags

KRAB, SV40 pA, and myc NLS

Expression

Mammalian

Mutation

Promoter

Alb-AAT

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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AatII-pRPS5A-PTP-3Xflag- psa A Right _G1397N

Plasmid

#189647

Purpose

psa A Right _G1397N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 H245N (AAT)

Plasmid

#212551

Purpose

Encodes SARS-CoV-2 spike H245N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike H245N (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

H245N

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 D950N (AAT)

Plasmid

#212425

Purpose

Encodes SARS-CoV-2 spike D950N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D950N (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

D950N

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 D936N (AAT)

Plasmid

#212405

Purpose

Encodes SARS-CoV-2 spike D936N for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike D936N (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

D936N

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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