We narrowed to 495 results for: ADK

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pTub-alpha1-Lifeact-GFP

Plasmid

#175437

Purpose

F-actin structures labelling

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

Lifeact-EGFP

Tags

EGFP

Expression

Mammalian

Promoter

pTub-alpha1

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pKJ237

Plasmid

#212320

Purpose

Express KnFnuc

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

KnFnuc

Expression

Bacterial

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ226

Plasmid

#212316

Purpose

Express DpFnuc with pureexpress

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc

Expression

Bacterial

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pTub-alpha1-iLifeact-NeonGreen

Plasmid

#175258

Purpose

F-actin structures labelling

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

iLifeact

Tags

mNeonGreen

Expression

Mammalian

Mutation

None (wt)

Promoter

pTub-alpha1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKJ248

Plasmid

#217494

Purpose

Mammlian expression of DpFnuc guide RNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc guide RNA scaffold

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ223

Plasmid

#217497

Purpose

Bacterial expression of BaFnuc guide RNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

BaFnuc guide RNA scaffold

Expression

Bacterial

Available Since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ224

Plasmid

#217496

Purpose

Bacterial expression of BaFnuc for IVTT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

BaFnuc

Expression

Bacterial

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ235

Plasmid

#217493

Purpose

Mammlian expression of MmFnuc for genome editing

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

MmFnuc

Expression

Mammalian

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAN052

Plasmid

#220046

Purpose

Mammalian expression of type III-A Csm complex from Streptococcus thermophilus without NLS tags

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

FLAG-Csm1; FLAG-Csm2; FLAG-Csm3; FLAG-Csm4; FLAG-Csm5; FLAG-Cas6; crRNA; GFP

Expression

Mammalian

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ318

Plasmid

#217495

Purpose

Mammlian expression of MmFnuc guide RNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

MmFnuc guide RNA scaffold

Expression

Mammalian

Available Since

April 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ234

Plasmid

#217492

Purpose

Mammlian expression of DpFnuc for genome editing

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

DpFnuc

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ221

Plasmid

#212319

Purpose

Express MmFnuc guide with pureexpress

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

Mercenaria guide

Expression

Bacterial

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ238

Plasmid

#212321

Purpose

Express KnFnuc guide with pureexpress

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

KnFunc guide

Expression

Bacterial

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKJ217

Plasmid

#212318

Purpose

Express MmFnuc with pureexpress

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

Insert

Mercenaria

Expression

Bacterial

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Expression

Mammalian

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2

Plasmid

#191320

Purpose

Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-Dronpa

Plasmid

#191321

Purpose

Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-Dronpa (Tubb2a Mouse)

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pTalpha1-iCre

Plasmid

#133924

Purpose

Neuron-specific expression of iCre

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

iCre

Expression

Mammalian

Mutation

Codon optimized

Promoter

neuron-specific tubulin-alpha-1 promoter

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-loxpSTOPloxp-lyn-NeonGreen

Plasmid

#175257

Purpose

membrane target protein labelling

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

Lyn membrane targeting motif

Use

Cre/Lox

Tags

mNeonGreen

Expression

Mammalian

Mutation

None (wt)

Promoter

pCAG

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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