We narrowed to 9,215 results for: Pol

1. pOliSpec3'

   Plasmid

   #228550

   Purpose

   Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.

   Depositor

   Jeferson Gross

   Insert

   Truncated 3' region of aadA
   

   Use

   CRISPR

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LvPolyUb

   Plasmid

   #218982

   Purpose

   L.variegatus polyubiquitin-C promoter

   Depositor

   Amro Hamdoun

   Insert

   L.variegatus polyubiquitinC promoter
   

   Use

   Sea urchin

   Promoter

   L.variegatus polyubiquitinC promoter

   Available Since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pssAAV_Seq17_CMV_bglobin_3NLS_TFP_WPRE_polyA

   Plasmid

   #220223

   Purpose

   AAV-STARR-seq validation control vector with TFP

   Depositor

   Simon Rumpel

   Article

   Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_Seq17_VS33_4.26_ChimeraIntron_3NLS_RFP_WPRE_polyA

   Plasmid

   #220225

   Purpose

   AAV-STARR-seq validation vector containing VS33 enhancer

   Depositor

   Simon Rumpel

   Article

   Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

   Insert

   Enhancer (VS33)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7CFE1_polio_3ABC_C147R

   Plasmid

   #212985

   Purpose

   Encodes Poliovirus type I Mahoney 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Poliovirus Type I Mahoney 3ABC C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Mutation

   cysteine 147 in 3C changed to arginine

   Promoter

   T7

   Available Since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7CFE1_polio_VP12A

   Plasmid

   #212988

   Purpose

   Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Poliovirus type I Mahoney VP12A
   

   Use

   Mammalian cell free protein expression

   Tags

   HA tag

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7CFE1_polio_VP12A_C109R

   Plasmid

   #212987

   Purpose

   Encodes Poliovirus type I Mahoney VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   Poliovirus Type I Mahoney VP12A C109R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Mutation

   Cysteine 109 from 2A mutated to arginine

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CL20_mEGFP_PICK1_POLR2F

   Plasmid

   #205894

   Purpose

   Express mEGFP-tagged fusion protein, PICK1_POLR2F from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   PICK1_POLR2F (PICK1, POLR2F Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CL20_mEGFP_TTC6_MIPOL1

   Plasmid

   #205949

   Purpose

   Express mEGFP-tagged fusion protein, TTC6_MIPOL1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   TTC6_MIPOL1 (TTC6, MIPOL1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cas9_P2A_T4pol

    Plasmid

    #190146

    Purpose

    Expresses SpCas9 and T4pol

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9_P2A_T4pol
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cas9_P2A_POLB

    Plasmid

    #190145

    Purpose

    Expresses SpCas9 and POLB

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9_P2A_POLB
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPOLY_new

    Plasmid

    #179276

    Purpose

    Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

    Inserts

    cellodextrin phosphorylase
    cellobiose phosphorylase
    sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
    

    Use

    Synthetic Biology

    Tags

    6xHIS and Strep-Tag II

    Expression

    Bacterial

    Promoter

    T7 lacO

    Available Since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLz58_polyPro_CsoS2_CTP

    Plasmid

    #140850

    Purpose

    Same as pLz47 but with C-terminal peptide (CTP) instead of N*- peptide

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 C-terminal peptide
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLz55_polyPro_random_Npeptide

    Plasmid

    #140846

    Purpose

    Same as pLz47 but with randomized N*-peptide sequence

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with randomized CsoS2 N-peptide
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLz56_polyPro_CsoS2_MR_r1

    Plasmid

    #140848

    Purpose

    Same as pLz47 but with MR repeat 1 instead of N*-peptide

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 MR repeat 1
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLz47_polyPro_CsoS2_Npeptide

    Plasmid

    #140845

    Purpose

    Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 N-peptide
    

    Tags

    Hexahistidine tag and Strep-tag II

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLz57_polyPro_CsoS2_CTD_r8

    Plasmid

    #140849

    Purpose

    Same as pLz47 but with CTD repeat 8 instead of N*-peptide

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    polyproline helix with CsoS2 CTD repeat 8
    

    Tags

    Hexahistidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PoliIFN-γ

    Plasmid

    #107289

    Purpose

    Plasmid can be used for secretion of fish Interferon gamma

    Depositor

    Bohdan Schneider

    Article

    Zahradnik et al Fish Shellfish Immunol. 2018 May 6;79:14

    Insert

    Interferon gamma
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-T7pol

    Plasmid

    #59926

    Purpose

    Expression vector for T7 RNA polymerase gene

    Depositor

    Ian Wickersham

    Insert

    T7 RNA polymerase
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOlyA(E69A)

    Plasmid

    #132392

    Purpose

    Mutant version of OlyA that senses sphingomyelin without or with cholesterol

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A point mutant (E69A)
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, E69A, C94S, S151C

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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