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Plasmid#86230PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.DepositorInsertAID[71-114]-eGFP
UseMouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct
Plasmid#156452PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTGDepositorInsertRAD21, AID (Rad21 Mouse)
UseMouse TargetingTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Unfused click editor construct; mSA-ePhi29 - pCMV-T7-mSA-Phi29 (JO1411)
Plasmid#217807PurposeUnfused click editor construct expressing mSA-ePhi29(D169A), expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPhi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Unfused click editor construct; PCV2-EcKlenow fusion - pCMV-T7-PCV2-EcKlenow (JO1421)
Plasmid#217804PurposeUnfused click editor construct expressing PCV2-EcKlenow, expressed from CMV or T7 promoters.DepositorInsertPCV2-linker-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationEcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
Unfused click editor construct; PCV2-ePhi29 fusion - pCMV-T7-PCV2-ePhi29 (JO1420)
Plasmid#217805PurposeUnfused click editor construct expressing PCV2-ePhi29(D169A), expressed from CMV or T7 promoters.DepositorInsertPCV2-linker-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationPhi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct
Plasmid#175549PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.DepositorInsertWAPL (Wapl Mouse)
UseMouse TargetingTagsAID[71-114]-eGFPExpressionMammalianMutationnonePromoterAvailable sinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Unfused click editor construct; mSA-EcKlenow fusion - pCMV-T7-mSA-EcKlenow (JO1401)
Plasmid#217806PurposeUnfused click editor construct expressing mSA-EcKlenow, expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationEcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)
Plasmid#213029PurposeExpresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2DepositorInsertHuman LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-JRGECO1a (AAV1)
Viral Prep#128317-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-Ef1a-fDIO-JRGECO1a (#128317). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO-JRGECO1a plasmid DNA. EF1a-driven, Flp-dependent expression of the biosensor jRGECO1a. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsNoneAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Cultivarium MACKEREL Library
Pooled Library#208054PurposepGL0_151 [RS421] is a library of broad host range prokaryotic promoters-RBS regulatory sequences formatted as a golden gate assembly Level 0 part.DepositorUseSynthetic BiologyAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 (AAV1)
Viral Prep#98928-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 (#98928). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent expression of Ruby2sm-Flag spaghetti monster construct. Spaghetti monster constructs are not fluorescent ("dark") to allow full range of antibody staining. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmRuby2sm-Flag (spaghetti monster construct)Available sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 (AAV1)
Viral Prep#98927-AAV1PurposeReady-to-use AAV1 particles produced from pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 (#98927). In addition to the viral particles, you will also receive purified pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent expression of GFPsm_myc spaghetti monster construct. Spaghetti monster constructs are not fluorescent ("dark") to allow full range of antibody staining. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPsm-myc (spaghetti monster construct)Available sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBh-mKate2-IRES-MCS (WT-WT) (AAV2)
Viral Prep#105921-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-CBh-mKate2-IRES-MCS (WT-WT) (#105921). In addition to the viral particles, you will also receive purified pAAV-CBh-mKate2-IRES-MCS (WT-WT) plasmid DNA. Control mKate2 expression AAV with two wild-type ITR sequences. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCBh and same CBh promoter as mKate2TagsmKate2Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pre-associated oBC-mBC backbone (p025)
Pooled Library#194097Purposepre-associated oBC-mBC backbone (p025), can insert additional reporter constructsDepositorExpressionBacterial and MammalianUseRNAiAvailable sinceApril 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human Kinase Domain Constructs for Automated Bacterial Expression
Plasmid Kit#1000000094Purpose68 His-tagged human kinase catalytic domain constructs and the respective phosphatases that enhance their bacterial expression; optimized for automated bacterial expressionDepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-BbChT (AAV9)
Viral Prep#45186-AAV9PurposeReady-to-use AAV9 particles produced from AAV-EF1a-BbChT (#45186). In addition to the viral particles, you will also receive purified AAV-EF1a-BbChT plasmid DNA. Brainbow construct encoding mCherry and mTFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmCherry, mTFP, or noneAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-BbTagBY (AAV9)
Viral Prep#45185-AAV9PurposeReady-to-use AAV9 particles produced from AAV-EF1a-BbTagBY (#45185). In addition to the viral particles, you will also receive purified AAV-EF1a-BbTagBY plasmid DNA. Brainbow construct encoding farnesylated TagBFP and EYFP, both in reverse orientation between mutant Lox sites. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsTagBFP, EYFP, or none.Available sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-tdTomato
Plasmid#239048PurposeAAV construct used as a cell filler to label neurons with tdTomatoDepositorInserttdTomato
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mTurquoise2
Plasmid#239206PurposeAAV construct used as a cell filler to label neurons with mTurquoise2DepositorInsertmTurquoise2
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
MW005 (bacterial strain)
Bacterial Strain#24545DepositorBacterial ResistanceStreptomycinAvailable sinceSept. 13, 2010AvailabilityAcademic Institutions and Nonprofits only