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Plasmid#177089PurposeMoClo Level P, position 1, plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT driven by 4x opaatBDepositorInsertCrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-PLA2G15-6xHis S198A
Plasmid#213605PurposeExpresses PLA2G15-6xHis S198A in mammalian cellsDepositorInsertPhospholipase A2 Group XV (PLA2G15 Human)
UseTags6xHisExpressionMammalianMutationChanged serine 198 to alaninePromoterCMVAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0792
Plasmid#177076PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31DepositorInsertmajor latex protein-like (NmMLPL) from Nepeta mussinii
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0120
Plasmid#177052PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii driven by 35S promoter; contains a chloroplast transit peptideDepositorInsertmajor latex protein-like (NmMLPL) from Nepeta mussinii
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-LpIA(wild-type)
Plasmid#61821PurposeEncodes a wild-type sequence of LplA and serves as the negative control for resorufin labelingDepositorInsertE. coli lipoic acid ligase
UseTags6XHis, EGFP, and FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVS1
Plasmid#33143DepositorInsertLacO (256 copies)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
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pPpT4- HTA1prom-TIR1-FLAG
Plasmid#189725PurposeEnables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)DepositorInsertOsTIR1
UseTagsFLAGExpressionYeastMutationPromoterHTA1Available sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
p35S::Cf-4-YFPc
Plasmid#86160PurposeCf-4 slplit YFPDepositorInsertCf-4
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1
Plasmid#164614PurposeLentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.DepositorInsertstdMCP-stdHalo-RH1
UseLentiviralTagsHA tag and NLSExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
NKP38 GFP (1009)
Plasmid#62021Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertAYTL1 (LPCAT2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV3Tag8li-hMBOAT7-3xFLAG
Plasmid#165474PurposeHuman MBOAT7 mammalian expression vector with 3 C-terminal FLAG tags.DepositorInsertMembrane bound O-acyltransferase domain containing 7 (MBOAT7 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA
Plasmid#105000PurposeExpresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
UseTags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVS1
Plasmid#33143DepositorInsertLacO (256 copies)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
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LD-GA
Plasmid#209862PurposeDimerization dependent fluorescent protein GA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddGFP A
UseTagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
LD-B
Plasmid#209861PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddGFP B
UseTagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
LD-RA
Plasmid#209863PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the lipid droplet membrane with hairpin domain of human GPAT4DepositorInsertddRFP A
UseTagsHairpin domain of GPAT4 MNFQYISLRLTVLWGLGVLIRYCFL…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- HTA1prom-TIR1-FLAG
Plasmid#189725PurposeEnables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)DepositorInsertOsTIR1
UseTagsFLAGExpressionYeastMutationPromoterHTA1Available sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
p35S::Cf-4-YFPc
Plasmid#86160PurposeCf-4 slplit YFPDepositorInsertCf-4
UseTagsYFPcExpressionPlantMutationPromoter35SAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only