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pAG499-FRB-N-frFAST

Plasmid

#160059

Purpose

Expresses FRB-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FRB-N-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Vrg4-V5-APEX2-URA

Plasmid

#207071

Purpose

Expressing Vrg4-V5-APEX2. Marker for late Golgi/early endosomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Vrg4

Tags

V5-APEX2

Expression

Yeast

Promoter

pVRG4

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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piiTRE-Bi-SG-Tii

Plasmid

#26121

Depositor

Liqun Luo

Article

Li et al PLoS One. 2010 . 5(7):e11503.

Insert

Synaptophysin (Syp Mouse)

Tags

mut4EGFP

Expression

Mammalian

Available Since

Nov. 1, 2010

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-TZAP

Plasmid

#87176

Purpose

retroviral stable expression of Flag-TZAP

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (ZBTB48 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2017

Availability

Academic Institutions and Nonprofits only

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hTRAF2K31RpLPCX-HA-Flag/P876

Plasmid

#20231

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

K31R (a92g in the TRAF2 CDS sequence).

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only
PABE-1

Plasmid

#115622

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

NLS-wtTadA-TadA7.10-nCas9-NLS

Use

CRISPR

Expression

Plant

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMN-326

Plasmid

#195451

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF3 BS - minTK - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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sv272-HisMBP-TZAPZNF9-11

Plasmid

#87185

Purpose

bacterial expression of HisMBP-TZAPZNF9-11

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (ZBTB48 Human)

Tags

HisMBP

Expression

Bacterial

Promoter

T7

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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Mammalian_SaAID3S

Plasmid

#188651

Purpose

Expresses SaAID3S and gRNA in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

nSaCas9-AID3S--UGI
Sa-sgRNA

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

EF-1a and H1

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Mammalian_SaAID2S

Plasmid

#188652

Purpose

Expresses SaAID2S and gRNA in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Inserts

AID2S-nSaCas9--UGI
Sa-sgRNA

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

EF-1a and H1

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-3×FLAG-SLC35A1

Plasmid

#186282

Purpose

transient expression of N-terminal 3×FLAG tagged SLC35A1 isoform a in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Li et al J Biol Chem. 2021 Sep;297(3):101069. doi

Insert

SLC35A1 (SLC35A1 Human)

Tags

3×FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC FLAG hRap1 delta Br delta M

Plasmid

#13256

Depositor

Titia de Lange

Article

Li et al Mol Biol Cell. 2003 Dec . 14(12):5060-8.

Insert

Repressor Activator Protein 1 delta Br delta M (TERF2IP Human)

Use

Retroviral

Tags

FLAG

Mutation

contains hRap1 aa2-44 + 184-399 and the point mut…

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pX330.iGFP1

Plasmid

#66581

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
PABE-2

Plasmid

#115623

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-nCas9-NLS

Use

CRISPR

Expression

Plant

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pX330.LoxP

Plasmid

#66586

Purpose

sgRNA for LSL

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LoxP sgRNA

Expression

Mammalian

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pLPC-Myc-TZAP

Plasmid

#87175

Purpose

retroviral stable expression of Myc-TZAP

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

TZAP (ZBTB48 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2017

Availability

Academic Institutions and Nonprofits only

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pX330.iGFP2

Plasmid

#66582

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pAG500-FKBP-N-frFAST

Plasmid

#160060

Purpose

Expresses FKBP-N-frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

FKBP-N-frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pX330.iGFP5

Plasmid

#66584

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Expression

Mammalian

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
pX330.LoxPO

Plasmid

#66585

Purpose

sgRNA for LSL

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

LSL-Tomato

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
Human NCKX4-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75212

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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PABE-3

Plasmid

#115624

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-NLS-nCas9-NLS

Use

CRISPR

Expression

Plant

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PABE-6

Plasmid

#115627

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

wtTadA-TadA7.10-nCas9-2*NLS

Use

CRISPR

Expression

Plant

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PABE-4

Plasmid

#115625

Purpose

Targeted A to G in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Li et al Genome Biol. 2018 May 29;19(1):59. doi:

Insert

NLS-nCas9-wtTadA-TadA7.10-NLS

Use

CRISPR

Expression

Plant

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330.iGFP3

Plasmid

#66583

Purpose

sgRNA for iGFP

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

iGFP

Expression

Mammalian

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-SYN1>Fas-Myc/Kir2.1*(E224G Y242F):P2A :dTomato

Plasmid

#242233

Purpose

Express Kir 2.1 and dTomato in the neurons

Depositor

Qingchun Tong

Article

Li et al Cell Rep. 2025 Jun 19;44(7):115863. doi:

Inserts

Fas
Myc/Kir2.1 (E224GY242F)

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

SYN

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pETA_21 tRNA genes

Plasmid

#242431

Purpose

Expresses 21 tRNAs. The plasmid contains a T7 promoter upstream and an Nt.BspQI recognition site as well as a T7 terminator downstream of each tRNA gene.

Depositor

Sebastian Maerkl

Article

Li et al Nat Commun. 2025 Jul 5;16(1):6212. doi:

Insert

21 tRNA genes

Expression

Bacterial

Available Since

Aug. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pSIn-H3K9ac BS

Plasmid

#235131

Purpose

H3K9ac FRET biosensor for real-time, single-cell monitoring of H3K9ac levels

Depositor

Qin Peng

Article

Li et al Med-X. 2024; 2, 26. doi: 10.1007/s44258-

Insert

BD1-Ypet-34mer-ECFP-H3

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSIn-H3K9ac Y97F BS

Plasmid

#235132

Purpose

Mutant (negative control) Y97F biosensor for pSin-H3K9ac Full

Depositor

Qin Peng

Article

Li et al Med-X. 2024; 2, 26. doi: 10.1007/s44258-

Insert

BD1Y97F-Ypet-34mer-ECFP-H3

Use

Lentiviral

Expression

Mammalian

Mutation

BD1Y97F

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLP4

Plasmid

#209959

Purpose

Entry vector to store Level 0* parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLP5

Plasmid

#209960

Purpose

Entry vector to store Level 0* resistance cassette parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLP6

Plasmid

#209961

Purpose

Entry vector to store Level 0* replication origin parts

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
ClhN-p500-Erg6-V5-APEX2-URA

Plasmid

#207070

Purpose

Expressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Erg6

Tags

V5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-p1k-OM14-V5-APEX2-Ura

Plasmid

#207073

Purpose

Expressing OM14-V5-APEX2. Marker for mitochondrial outer membrane.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

OM14

Tags

V5-APEX2

Expression

Yeast

Promoter

pOM14

Available Since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-lyn10-hLOV-ssrA-P2A-LOVPLD-mCherry-sspB

Plasmid

#219557

Purpose

PM-targeted iLID-LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

lyn10-iLID-LOVPLD

Tags

mCherry

Expression

Mammalian

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-p1k-Cox4-V5-APEX2-Ura

Plasmid

#207061

Purpose

Expressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

V5-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-p1k-Cox4-GFP-APEX2-Ura

Plasmid

#207062

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Tags

GFP-APEX2

Expression

Yeast

Promoter

pCOX4

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-pADH3-Snf7-4V5-APEX2-URA

Plasmid

#207065

Purpose

Moderate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Snf7

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pADH3

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-pERG6-Pex3-4V5-APEX2-URA

Plasmid

#207068

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pERG6

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ClhN-pLSP1-Pex3-4V5-APEX2-URA

Plasmid

#207069

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Tags

4xV5-APEX2

Expression

Yeast

Promoter

pLSP1

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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