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pUDE1084

Plasmid

#168500

Purpose

expression of T7RNA polymerase and CRISPR endonunclease Fncas12a

Depositor

Jean-Marc Daran

Article

Randazzo et al CRISPR J. 2021 Apr 23. doi: 10.1089/cris

Inserts

Fncas12a
T7RNA polymerase

Use

CRISPR

Promoter

TDH3 and TEF1

Available Since

Dec. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniSOG

Plasmid

#193913

Purpose

Control plasmid that expresses non-targeted miniSOG in mammalian cells

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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MCV-Rep-

Plasmid

#32058

Depositor

Patrick Moore

Article

Feng et al PLoS One. 2011;6(7):e22468. Epub 2011 Ju

Insert

MCV-Rep- genome

Mutation

Single cytosine to adenine (C/A) mutation at 2,07…

Promoter

none from vector. Insert contains native promoter…

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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KA501: pMVP (L3-L2) pA; CMV::TETa-pA

Plasmid

#121801

Purpose

pMVP L3-L2 entry plasmid, contains polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven TETa downstream of gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

polyA + CMV::TETa-polyA

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCfb13198

Plasmid

#219873

Purpose

The base plasmid of TUNEYALI forTF08

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF08 ( YALI1_D33640g) and homologous arm matching TF08

Expression

Yeast

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb12567

Plasmid

#219927

Purpose

PrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter CYC

Expression

Yeast

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb12566

Plasmid

#219926

Purpose

PrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter DGA

Expression

Yeast

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13236

Plasmid

#219909

Purpose

The base plasmid of TUNEYALI for TF46

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF46 ( YALI1_E20229g) and homologous arm matching TF46

Expression

Yeast

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13199

Plasmid

#219874

Purpose

The base plasmid of TUNEYALI forTF09

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF09 (YALI1_F29652g) and homologous arm matching TF09

Expression

Yeast

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13218

Plasmid

#219893

Purpose

The base plasmid of TUNEYALI for TF28

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF28 (YALI1_B28150g) and homologous arm matching TF28

Expression

Yeast

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCfb13239

Plasmid

#219912

Purpose

The base plasmid of TUNEYALI for TF49

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49

Expression

Yeast

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13247

Plasmid

#219918

Purpose

The base plasmid of TUNEYALI for TF57

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF57 (YALI1_D17249g) and homologous arm matching TF57

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13228

Plasmid

#219903

Purpose

The base plasmid of TUNEYALI for TF38

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF38 (YALI1_A14345g) and homologous arm matching TF38

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13210

Plasmid

#219885

Purpose

The base plasmid of TUNEYALI for TF20

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20

Expression

Yeast

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pWzyE-His

Plasmid

#196669

Purpose

Expression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Tags

TEV cleavable His10

Expression

Bacterial

Promoter

araBAD

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13220

Plasmid

#219895

Purpose

The base plasmid of TUNEYALI for TF30

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF30 (YALI1_D10007g) and homologous arm matching TF30

Expression

Yeast

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12622

Plasmid

#219929

Purpose

PrACT with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter ACT

Expression

Yeast

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13237

Plasmid

#219910

Purpose

The base plasmid of TUNEYALI for TF47

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF47 (YALI1_C19151g) and homologous arm matching TF47

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12562

Plasmid

#219923

Purpose

PrGPD with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter GPD

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13186

Plasmid

#219924

Purpose

PrTEF with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter TEF

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13204

Plasmid

#219879

Purpose

The base plasmid of TUNEYALI for TF14

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF14 (YALI1_F21261g) and homologous arm matching TF14

Expression

Yeast

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13200

Plasmid

#219875

Purpose

The base plasmid of TUNEYALI forTF10

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF10 ( YALI1_C16204g) and homologous arm matching TF10

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13242

Plasmid

#219914

Purpose

The base plasmid of TUNEYALI for TF52

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF52 (YALI1_E12668g) and homologous arm matching TF52

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13245

Plasmid

#219916

Purpose

The base plasmid of TUNEYALI for TF55

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF55 (YALI1_A16891g) and homologous arm matching TF55

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13193

Plasmid

#219868

Purpose

The base plasmid of TUNEYALI for TF03

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF03 (YALI1_B05350g) and homologous arm matching TF03

Expression

Yeast

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12561

Plasmid

#219922

Purpose

PrPGM with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter PGM

Expression

Yeast

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb12565

Plasmid

#219925

Purpose

PrFBA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Promoter FBA

Expression

Yeast

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13223

Plasmid

#219898

Purpose

The base plasmid of TUNEYALI for TF33

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF33 (YALI1_F14650g) and homologous arm matching TF33

Expression

Yeast

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13197

Plasmid

#219872

Purpose

The base plasmid of TUNEYALI forTF07

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF07 ( YALI1_D30097g) and homologous arm matching TF07

Expression

Yeast

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCfb13226

Plasmid

#219901

Purpose

The base plasmid of TUNEYALI for TF36

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF36 (YALI1_A22330g) and homologous arm matching TF36

Expression

Yeast

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfb13194

Plasmid

#219869

Purpose

The base plasmid of TUNEYALI for TF04

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF04 (YALI1_C02831g) and homologous arm matching TF04

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfb13209

Plasmid

#219884

Purpose

The base plasmid of TUNEYALI for TF19

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF19 (YALI1_A20855g) and homologous arm matching TF19

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfb13208

Plasmid

#219883

Purpose

The base plasmid of TUNEYALI for TF18

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF18 (YALI1_D15792g) and homologous arm matching TF18

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfb13207

Plasmid

#219882

Purpose

The base plasmid of TUNEYALI for TF17

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF17 (YALI1_B19962g) and homologous arm matching TF17

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfb13244

Plasmid

#219915

Purpose

The base plasmid of TUNEYALI for TF54

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF54 (YALI1_F26367g) and homologous arm matching TF54

Expression

Yeast

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfb13233

Plasmid

#219906

Purpose

The base plasmid of TUNEYALI for TF43

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF43 (YALI1_C14774g) and homologous arm matching TF41

Expression

Yeast

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfb13206

Plasmid

#219881

Purpose

The base plasmid of TUNEYALI for TF16

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF16 (YALI1_B11759g) and homologous arm matching TF16

Expression

Yeast

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfb13196

Plasmid

#219871

Purpose

The base plasmid of TUNEYALI forTF06

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF06 (YALI1_D34785g) and homologous arm matching TF06

Expression

Yeast

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfb13217

Plasmid

#219892

Purpose

The base plasmid of TUNEYALI for TF27

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF27 (YALI1_E22758g) and homologous arm matching TF27

Expression

Yeast

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfb13231

Plasmid

#219905

Purpose

The base plasmid of TUNEYALI for TF41

Depositor

Irina Borodina

Article

Jiang et al Proc Natl Acad Sci U S A. 2025 Jun 10;12

Insert

Contains gRNA targeting TF41 (YALI1_D00747g) and homologous arm matching TF40

Expression

Yeast

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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