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Plasmid#204573PurposeExpresses Ash-tagged LC3B in mammalian cellsDepositorInsertLC3B (MAP1LC3B Human)
UseTagsExpressionMammalianMutationdelta121-125aaPromoterAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3B Q116P G120
Plasmid#129291PurposeGateway entry clone encoding human deconjugation-resistant LC3BDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseGateway entry vector / entry cloneTagsExpressionMutationChanged Glutamine 116 to Proline. Deleted amino a…PromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTagGFP2-N SIN1 delta95-114
Plasmid#124925PurposeFor expression of a.a.95-114 deletion mutant of SIN1-GFPDepositorInsertMAPKAP1 (MAPKAP1 Human)
UseTagsTagGFP2ExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTagGFP2-N SIN1 delta116-127
Plasmid#124926PurposeFor expression of a.a.116-127 deletion mutant of SIN1-GFPDepositorInsertMAPKAP1 (MAPKAP1 Human)
UseTagsTagGFP2ExpressionMammalianMutationInsert ORF was sirently mutated to be resistent t…PromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP
Plasmid#216479Purposelentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excisionDepositorInsertsUseCre/Lox and LentiviralTagsExpressionMutationPromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro
Plasmid#216249PurposeExpress Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminusDepositorInsertCas9 and sgRANGAP.C1 (RANGAP1 Human)
UseCRISPR; Endogenous taggingTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV GFP-LC3B Q116P G120
Plasmid#129290PurposeExpresses GFP-tagged deconjugation-resistant LC3B in mammalian cellsDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTagsGFPExpressionMammalianMutationChanged Glutamine 116 to Proline. Deleted amino a…PromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF1932
Plasmid#142292PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertJUN (JUN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-JUN
Plasmid#192895PurposeBarcoded piggybac transposon vector with Dox-inducible expression of JUNDepositorInsertJUN (JUN Human)
UseTagsHAExpressionMammalianMutationPromoterTET3G-miniCMVAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA-humanTRAF3 (1098)
Plasmid#66927PurposeMammalian Expression of TRAF3DepositorInsertTRAF3 (TRAF3 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag-humanTRAF3 (1096)
Plasmid#66928PurposeMammalian Expression of TRAF3DepositorInsertTRAF3 (TRAF3 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGR475
Plasmid#213784PurposeIntegrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance markerDepositorInsertWT TP-DNAP1 (recoded)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
1553 pcDNA3 HA gamma adaptin 2
Plasmid#10713DepositorInsertGamma adaptin 2 (AP1G2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLU-CMV-PL3 Flag-humanTRAF3-WT (1114)
Plasmid#66922PurposeLentiviral Expression of TRAF3DepositorInsertTRAF3 (TRAF3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGR595
Plasmid#213785PurposeIntegrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.DepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG2-EGFP-C-IFT121
Plasmid#218736PurposeExpresses C-terminally EGFP-tagged IFT121 in mammalian cellsDepositorInsertIFT121 (WDR35 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCAGAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pGR596
Plasmid#213787PurposeIntegrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationSee manuscriptPromoterAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only